Description

Phosphoenol-3-Bromopyruvate CAS NO 44976-95-6 is a specialized brominated derivative of phosphoenolpyruvate, a key intermediate in glycolysis and gluconeogenesis. This compound is of significant interest as a potent and selective inhibitor of the M2 isoform of pyruvate kinase (PKM2), a metabolic enzyme frequently upregulated in cancer cells. It serves as a critical biochemical tool for researchers in oncology, metabolic disease, and enzymology, enabling the study of the Warburg effect and tumor metabolism. Pharmaceutical and biotechnology companies utilize this high-purity intermediate in the development of novel therapeutic agents and diagnostic probes.

Application

• Biochemical Research: As a selective inhibitor of pyruvate kinase M2 (PKM2) for studying cancer cell metabolism, the Warburg effect, and metabolic reprogramming.
• Pharmaceutical Development: A key intermediate and pharmacophore in the synthesis of potential anti-cancer therapeutics targeting metabolic pathways.
• Enzymology Studies: Used to investigate enzyme kinetics, allosteric regulation, and the role of PKM2 in cellular signaling beyond glycolysis.
• Diagnostic Probe Synthesis: Serves as a precursor for developing molecular probes and imaging agents for detecting PKM2 activity in vitro and in vivo.
• Metabolic Pathway Analysis: A tool for modulating glycolytic flux in cell cultures and model organisms to understand metabolic disorders.
• Academic & Institutional Research: Fundamental research in biochemistry, molecular biology, and chemical biology at universities and research institutes.

Basic Information

Product Name	Phosphoenol-3-Bromopyruvate
CAS No.	44976-95-6
Molecular Formula	C3H2BrO6P
Molecular Weight	244.92 g/mol
Synonyms	3-Bromophosphoenolpyruvate; 3-Bromo-PEP; (Z)-3-Bromo-2-(phosphonooxy)acrylic acid; Bromopyruvate phosphoenol ester; PKM2 Inhibitor; 3-Bromopyruvic acid phosphate enol ester; 2-(Phosphonooxy)-3-bromo-2-propenoic acid
EINECS	Contact for details

Quality Control

Our Phosphoenol-3-Bromopyruvate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and MS, to ensure it meets the high standards required for research and development applications. Certificates of Analysis (COA) with detailed chromatographic data are provided for every shipment, ensuring traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Keep container tightly sealed in a desiccator after opening.

Specification

Item	Specification
Appearance	White to off-white powder or crystalline solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.