Description

Biotinyl Tyramide is a high-purity biochemical reagent widely used as a critical intermediate and amplification reagent in advanced molecular detection systems. Its core value lies in enabling highly sensitive signal amplification in techniques like Tyramide Signal Amplification (TSA), which is essential for detecting low-abundance targets in research and diagnostics. This compound is primarily needed by biotechnology, pharmaceutical R&D, and in vitro diagnostic (IVD) kit manufacturers developing next-generation assays for genomics, proteomics, and immunohistochemistry.

Application

• Tyramide Signal Amplification (TSA/CSA): A key reagent for catalyzed reporter deposition (CARD) to dramatically enhance detection sensitivity in immunohistochemistry (IHC), in situ hybridization (ISH), and immunofluorescence.
• Molecular Diagnostics: Used in the development of high-sensitivity diagnostic assays and kits for detecting biomarkers, pathogens, and genetic mutations.
• Bioconjugation & Probe Labeling: Serves as an intermediate for conjugating biotin to antibodies, oligonucleotides, or other proteins to create detection probes.
• Life Science Research: Enables sensitive detection of nucleic acids and proteins in blotting applications (Western, Northern, Southern) and microarray analysis.
• Pharmaceutical Development: Utilized in drug discovery for target validation and high-content screening assays requiring ultra-sensitive readouts.
• IVD Manufacturing: A critical raw material for in vitro diagnostic reagent producers formulating advanced staining and detection systems.

Basic Information

Product Name	Biotinyl Tyramide
CAS No.	41994-02-9
Molecular Formula	C18H25N3O4S
Molecular Weight	379.48 g/mol
Synonyms	Biotin Tyramide; Biotinylated Tyramide; N-(3-(4-Hydroxyphenyl)propanoyl)biocytin; Tyramide-Biotin; Biotinyl Tyramine; TSA Biotin; Biotin-XX Tyramide; Catalyzed Signal Amplification (CSA) Reagent
EINECS	Contact for details

Quality Control

Our Biotinyl Tyramide CAS NO 41994-02-9 is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the stringent requirements of research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. For long-term storage, consider desiccated conditions at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.