Description

n-Benzyloxycarbonylglycyl-Leucyl-Phenylalanine Chloromethyl Ketone is a specialized peptide-derived chloromethyl ketone, a potent and irreversible inhibitor of serine proteases. This compound is critical for biochemical research and pharmaceutical development, enabling precise study and modulation of enzymatic activity. It is primarily utilized by researchers in proteomics, medicinal chemistry, and drug discovery for investigating disease mechanisms and developing targeted therapeutic agents.

Application

• Protease Inhibition Research: Acts as a specific, irreversible active-site inhibitor for studying serine protease function and kinetics.
• Medicinal Chemistry & Drug Discovery: Serves as a key pharmacophore or building block in the design of novel therapeutic agents targeting protease-related diseases.
• Biochemical Assay Development: Used to develop and validate high-throughput screening assays for identifying new protease inhibitors.
• Apoptosis & Cell Signaling Studies: Employed to investigate the role of specific proteases in programmed cell death and signal transduction pathways.
• Enzyme Mechanism Elucidation: A vital tool for structural biologists and enzymologists to probe catalytic mechanisms and active site architecture.
• Pharmaceutical Intermediate: Functions as a sophisticated synthetic intermediate in the multi-step production of complex bioactive molecules.

Basic Information

Product Name	n-Benzyloxycarbonylglycyl-Leucyl-Phenylalanine Chloromethyl Ketone
CAS No.	41658-44-0
Molecular Formula	C29H36N3O6Cl
Molecular Weight	558.07 g/mol
Synonyms	Z-Gly-Leu-Phe-CH2Cl; Z-Gly-Leu-Phe-CMK; Benzyloxycarbonyl-Gly-Leu-Phe-chloromethyl ketone; Cbz-Gly-Leu-Phe-CH2Cl; N-Carbobenzoxy-glycyl-leucyl-phenylalanine chloromethyl ketone; Protease Inhibitor Z-GLF-CMK; (S)-1-(((S)-1-(((S)-4-((Benzyloxy)carbonylamino)-1-chloro-4-oxobutan-2-yl)amino)-4-methyl-1-oxopentan-2-yl)amino)-3-phenyl-1-oxopropan-2-yl)carbamate
EINECS	Contact for details

Quality Control

Our n-Benzyloxycarbonylglycyl-Leucyl-Phenylalanine Chloromethyl Ketone is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity and identity confirmation via spectroscopic methods. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all critical quality attributes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and potency. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in alcohols

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.