Description

(20R,14β,17β)-5α-Cholestane is a high-purity sterane compound, a saturated derivative of cholesterol that serves as a critical reference standard and biochemical building block. Its defined stereochemistry makes it an essential tool for analytical calibration and research into lipid metabolism and membrane biophysics. This compound is primarily utilized by pharmaceutical R&D laboratories, analytical testing facilities, and academic institutions engaged in steroid and natural product chemistry.

Application

• Analytical Reference Standard: Used as a primary standard in Gas Chromatography (GC) and High-Performance Liquid Chromatography (HPLC) for the identification and quantification of sterols and related compounds in complex matrices.
• Biochemical Research: Serves as a key intermediate or model compound in studies of cholesterol biosynthesis, metabolism, and the structural properties of lipid membranes.
• Pharmaceutical Development: Employed in the synthesis and quality control of steroid-based active pharmaceutical ingredients (APIs) and in metabolic pathway analysis.
• Geochemical Biomarker: Acts as a stable biomarker in petroleum geochemistry and environmental studies for tracing organic matter origin and maturity.
• Nutritional Science: Used in research focused on sterol absorption, metabolism, and their impact on human health.

Basic Information

Product Name	(20R,14β,17β)-5α-Cholestane
CAS No.	40071-70-3
Molecular Formula	C₂₇H₄₈
Molecular Weight	372.67 g/mol
Synonyms	5α-Cholestane; Cholestane; (5α)-Cholestane; 5α-Cholestan; NSC 9257; 5α-Cholestane (8CI); allocholestane; Coprostane; Dihydrocholesterol (hydrogenated)
EINECS	254-754-8

Quality Control

Our (20R,14β,17β)-5α-Cholestane is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including chromatographic purity assays and identity confirmation, to ensure it meets the stringent requirements for use as a reference material. A detailed Certificate of Analysis (COA) providing batch-specific data for identity, purity, and related substances is supplied with every order.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is stable under these conditions for extended periods.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC/GC)	≥ 98.0%
Melting Point	79 - 82 °C
Specific Rotation	Contact for details
Loss on Drying	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.