Description

(-)-Cotinine CAS NO 486-56-6 is the principal metabolite of nicotine, known for its high purity and stability. This compound serves as a critical biomarker for nicotine exposure and a valuable reference standard in analytical and clinical research. It is essential for pharmaceutical R&D, diagnostic assay development, and toxicology studies across the global life sciences sector.

Application

• Biomarker & Reference Standard: Primary metabolite used as a definitive analytical standard for quantifying nicotine consumption in clinical, forensic, and public health research.
• Pharmaceutical Research: Key intermediate and active metabolite studied in the development of smoking cessation therapies and neurological agents.
• Diagnostic Assay Development: Critical component in the calibration and validation of immunoassays and LC-MS/MS methods for nicotine exposure testing.
• Toxicology & Pharmacokinetics: Used in studies to understand the metabolism, distribution, and long-term effects of nicotine in the body.
• Academic & Clinical Studies: Fundamental reagent for investigating the pharmacological and behavioral effects of nicotine and its metabolites.

Basic Information

Item	Details
Product Name	(-)-Cotinine
CAS No.	486-56-6
Molecular Formula	C10H12N2O
Molecular Weight	176.22 g/mol
Synonyms	(S)-(-)-Cotinine; (5S)-1-Methyl-5-(3-pyridinyl)-2-pyrrolidinone; Cotinine; L-(-)-Cotinine; (-)-(S)-Cotinine; Nicotine Metabolite; Cotinin; NSC 407098; 2-Pyrrolidinone, 1-methyl-5-(3-pyridinyl)-, (5S)-
EINECS	207-634-9

Quality Control

Our (-)-Cotinine is produced under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive analytical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles. We support compliance with GMP and ISO guidelines for reference materials, and specifications can be tailored to meet specific pharmacopeial (e.g., USP) or customer requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). The product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Specific Rotation [α]20/D	-84° to -90° (c=1 in H2O)
Related Substances (HPLC)	Total impurities ≤ 2.0%
Residual Solvents (GC)	Complies with ICH guidelines
Loss on Drying	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.