Description

Tpck CAS NO 402-71-1 is a highly specific, irreversible inhibitor of serine proteases, most notably chymotrypsin. This compound is a critical tool for biochemical research, enabling the selective blocking of enzymatic activity to study protein function and pathways. It is essential for researchers and manufacturers in the fields of proteomics, pharmaceutical development, and diagnostic reagent production who require precise enzyme control.

Application

• Protease Inhibition Studies: Used as a specific active-site titrant and inhibitor for chymotrypsin and chymotrypsin-like serine proteases in mechanistic enzymology.
• Biopharmaceutical R&D: Employed in the purification and characterization of therapeutic proteins and antibodies to prevent proteolytic degradation during processing.
• Cell Biology & Proteomics: A key reagent for dissecting protease-dependent signaling pathways and preparing cell lysates free of specific proteolytic activity.
• Diagnostic Manufacturing: Utilized in the production and quality control of diagnostic kits that rely on specific enzyme-substrate interactions.
• Structural Biology: Aids in crystallizing proteins by irreversibly inhibiting contaminating proteases, leading to more stable protein samples.
• Food Science Research: Applied in studies investigating the role of specific proteases in food texture, flavor development, and spoilage.

Basic Information

Product Name	Tpck
CAS No.	402-71-1
Molecular Formula	C14H14ClNO2S
Molecular Weight	295.78 g/mol
Synonyms	N-Tosyl-L-phenylalanine chloromethyl ketone; TPCK; L-1-Chloro-3-tosylamido-4-phenyl-2-butanone; TLCK (common confusion with TLCK, but they are different inhibitors); Chymotrypsin Inhibitor; N-p-Tosyl-L-phenylalanyl chloromethane; Tosylphenylalanyl chloromethyl ketone; Nα-Tosyl-L-phenylalanine chloromethyl ketone
EINECS	206-951-4

Quality Control

Our Tpck is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and identity confirmation by spectroscopic methods, to ensure it meets the high standards required for life science research. Certificates of Analysis (COA) with detailed chromatographic data are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Specific Rotation	-50° to -55° (c=1 in DMF)
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.