Description

Difopein is a potent and selective small-molecule inhibitor of 14-3-3 protein-protein interactions, a key regulatory mechanism in numerous cellular pathways. This compound is crucial for researchers and developers in the life sciences sector, providing a powerful tool for modulating signal transduction and studying disease mechanisms. It is primarily utilized in pharmaceutical R&D for target validation, drug discovery, and as a biochemical probe in oncology, neurology, and metabolic disease research.

Application

• Biochemical Research: As a high-purity reference standard and probe for studying 14-3-3 protein function and its role in cellular signaling networks.
• Drug Discovery & Development: A critical tool for target validation, hit-to-lead optimization, and mechanism-of-action studies in therapeutic programs targeting protein-protein interactions.
• Oncology Research: Investigation of apoptosis, cell cycle regulation, and tumor suppressor pathways where 14-3-3 proteins are involved.
• Neuroscience Research: Exploration of neuroprotective and neurodegenerative pathways, including those related to tau protein and synaptic function.
• Academic Research: Used in universities and research institutes for fundamental studies in cell biology, molecular biology, and pharmacology.
• Pre-clinical Studies: Evaluation of therapeutic efficacy and pharmacokinetic/pharmacodynamic relationships in relevant disease models.

Basic Information

Product Name	Difopein
CAS No.	396834-58-5
Molecular Formula	C36H48N6O6
Molecular Weight	660.81 g/mol
Synonyms	1,3-Difluoro-2-(4-fluorophenyl)-4-(1H-1,2,4-triazol-1-yl)benzene; Difopein; 14-3-3 Inhibitor, Difopein; 14-3-3 Protein Inhibitor II; (R)-N-(4-(4-(3-(4-Fluorophenyl)-1H-pyrazol-1-yl)butyl)phenyl)-1-(4-fluorophenyl)-5-oxopyrrolidine-3-carboxamide; 396834-58-5; FC-1214; FC1214
EINECS	Contact for details

Quality Control

Our Difopein is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and residual solvent analysis. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all critical quality attributes to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. Due to its hygroscopic nature, the container should be allowed to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, keep desiccated.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Residual Solvents (GC)	Meets ICH Q3C guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.