Description

Sirolimus-D3 (Rapamycin-D3) is a deuterated analog of the potent immunosuppressant and mTOR inhibitor, Sirolimus, where three hydrogen atoms are replaced by deuterium. This stable isotope-labeled compound is essential for use as an internal standard in quantitative bioanalytical methods, ensuring the highest accuracy and reliability in pharmacokinetic and metabolic studies. It is a critical tool for pharmaceutical R&D, clinical research, and forensic toxicology laboratories requiring precise measurement of Sirolimus levels in complex biological matrices.

Application

• Bioanalytical Internal Standard: Primary use as a deuterated internal standard for Liquid Chromatography-Mass Spectrometry (LC-MS/MS) quantification of Sirolimus in plasma, serum, and tissue samples.
• Pharmacokinetic & Metabolism Research: Enables accurate tracking of drug absorption, distribution, metabolism, and excretion (ADME) in preclinical and clinical studies.
• Therapeutic Drug Monitoring (TDM): Critical for developing and validating robust assays to monitor Sirolimus blood levels in transplant patients to optimize efficacy and minimize toxicity.
• Drug Discovery & Development: Used in high-throughput screening and mechanistic studies to understand the pharmacokinetic profile of new mTOR-targeting therapeutics.
• Forensic & Doping Analysis: Provides definitive quantification in forensic investigations or anti-doping controls where Sirolimus may be a substance of interest.
• Manufacturing Quality Control: Serves as a reference standard in the quality control of Sirolimus active pharmaceutical ingredient (API) and finished drug products.

Basic Information

Product Name	Sirolimus-D3 / Rapamycin-D3
CAS No.	392711-19-2
Molecular Formula	C51H76D3NO13
Molecular Weight	~ 917.2 g/mol
Synonyms	Rapamycin-d3; Deuterated Rapamycin; Sirolimus (methyl-d3); AY 22989-d3; NSC 226080-d3; (3S,6R,7E,9R,10R,12R,14S,15E,17E,19E,21S,23S,26R,27R,34aS)-9,10,12,13,14,21,22,23,24,25,26,27,32,33,34,34a-Hexadecahydro-9,27-dihydroxy-3-[(1R)-2-[(1S,3R,4R)-4-hydroxy-3-methoxycyclohexyl]-1-methylethyl]-10,21-dimethoxy-6,8,12,14,20,26-hexamethyl-23,27-epoxy-3H-pyrido[2,1-c][1,4]oxazacyclohentriacontine-1,5,11,28,29(4H,6H,31H)-pentone-d3
EINECS	Contact for details

Quality Control

Our Sirolimus-D3 is manufactured and handled under strict quality systems to ensure the integrity required for analytical reference standards. Each batch is characterized using advanced spectroscopic and chromatographic techniques, including NMR, HPLC, and MS, to confirm isotopic enrichment, chemical purity, and identity. A comprehensive Certificate of Analysis (COA) is provided, detailing batch-specific data. We support compliance needs for research conducted under GLP (Good Laboratory Practice) and GMP (Good Manufacturing Practice) guidelines.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This compound is hygroscopic (moisture-sensitive); ensure containers are sealed under inert atmosphere after each use to maintain stability and purity. For long-term storage, desiccants are recommended.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Isotopic Purity (Deuterium Content)	≥ 98 atom % D
Chemical Purity (HPLC)	≥ 98.0%
Specific Optical Rotation	Contact for details
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.