Description

7-Dehydrocholesterol-25,26,26,26,27,27,27-D7 is a high-purity, deuterium-labeled analog of 7-dehydrocholesterol, a key intermediate in the biosynthesis of vitamin D3. This stable isotope-labeled compound is essential for use as an internal standard in quantitative mass spectrometry, ensuring unparalleled accuracy and reliability in analytical measurements. It is critically needed by pharmaceutical R&D laboratories, clinical research organizations, and analytical service providers for method development, pharmacokinetic studies, and quality control of vitamin D-related metabolites.

Application

• Internal Standard for LC-MS/MS: Primary application for the precise quantification of endogenous 7-dehydrocholesterol and related sterols in complex biological matrices like serum, plasma, and tissues.
• Metabolic Pathway Tracing: Used in deuterium-labeling studies to investigate the biosynthesis and metabolic fate of vitamin D3 and its precursors.
• Pharmaceutical R&D: Supports drug discovery and development programs targeting disorders of cholesterol and vitamin D metabolism.
• Clinical Diagnostics: Enables the development and validation of high-sensitivity assays for diagnosing inborn errors of metabolism, such as Smith-Lemli-Opitz syndrome.
• Biomarker Research: Facilitates accurate measurement of sterol biomarkers in epidemiological and nutritional studies.
• Reference Material: Serves as a certified reference standard for calibrating analytical instruments and ensuring regulatory compliance (FDA, EMA).

Basic Information

Product Name	7-Dehydrocholesterol-25,26,26,26,27,27,27-D7
CAS No.	388622-58-0
Molecular Formula	C27H37D7O
Molecular Weight	387.66 g/mol (approx.)
Synonyms	(3β)-Cholesta-5,7-dien-3-ol-25,26,26,26,27,27,27-d7; 7-DHC-d7; Provitamin D3-d7; (3S,10R,13R,17R)-10,13-Dimethyl-17-[(2R)-6-methylheptan-2-yl]-2,3,4,9,11,12,14,15,16,17-decahydro-1H-cyclopenta[a]phenanthren-3-ol-d7; 5,7-Cholestadien-3β-ol-d7; Deuterated 7-Dehydrocholesterol
EINECS	Contact for details

Quality Control

Every batch of 7-Dehydrocholesterol-25,26,26,26,27,27,27-D7 is manufactured and analyzed under strict quality systems. We guarantee high chemical and isotopic purity, with comprehensive testing performed via NMR, HPLC, and mass spectrometry. Certificates of Analysis (COA) are provided with each shipment, detailing batch-specific results for identity, purity, isotopic enrichment, and solvent residues, supporting compliance with cGMP and ISO 17025 standards for reference materials.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) to prevent oxidation. For long-term storage, keep the primary container in a sealed secondary container with desiccant. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity (NMR)	Conforms
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.