Description

Cholestane-2,2,3,3,4,4-D6 is a high-purity, deuterium-labeled analog of cholestane, a key steroidal hydrocarbon. This stable isotope-labeled compound is essential as an internal standard in quantitative mass spectrometry, ensuring analytical accuracy and reproducibility. It is primarily required by analytical laboratories, pharmaceutical R&D departments, and contract research organizations (CROs) engaged in lipidomics, metabolomics, and steroid hormone analysis.

Application

• Internal Standard for Mass Spectrometry (MS): Critical for the precise quantification of cholesterol and related sterols in biological matrices via GC-MS or LC-MS/MS.
• Biomedical Research: Used in metabolic pathway studies, biomarker discovery, and investigations into lipid metabolism disorders.
• Pharmaceutical Development: Supports pharmacokinetic (PK) and bioanalytical studies for drug candidates targeting lipid pathways.
• Clinical Diagnostics: Enables accurate measurement of sterol levels in clinical samples for disease diagnosis and monitoring.
• Food & Nutrition Science: Applied in the analysis of sterol content and composition in food products and dietary supplements.
• Isotope Dilution Analysis (IDA): Serves as the labeled spike in high-accuracy IDA methods to correct for sample preparation and instrument variability.

Basic Information

Product Name	Cholestane-2,2,3,3,4,4-D6
CAS No.	358731-18-7
Molecular Formula	C₂₇H₄₂D₆
Molecular Weight	378.69 g/mol
Synonyms	Cholestane-d6 (2,2,3,3,4,4); 5α-Cholestane-2,2,3,3,4,4-d6; (5α)-Cholestane-2,2,3,3,4,4-d6; Cholestane-2,2,3,3,4,4-hexadeuterio; Deuterated Cholestane; Cholestane Internal Standard-d6; 5α-Cholestane-d6; NSC 18188-d6
EINECS	Contact for details

Quality Control

Every batch of Cholestane-2,2,3,3,4,4-D6 is produced and tested to meet the stringent requirements for isotopic purity and chemical purity demanded by advanced analytical applications. Our quality assurance includes rigorous analysis by NMR (¹H, ¹³C) and mass spectrometry to confirm isotopic enrichment and identity. A comprehensive Certificate of Analysis (COA) detailing purity, isotopic abundance, and analytical results is provided with each shipment to ensure full traceability and compliance with your research protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline solid
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0% (chemical purity)
Isotopic Purity (MS)	≥ 98.0 atom % D
Isotopic Enrichment	≥ 99% at labeled positions (2,2,3,3,4,4-D6)
Water Content (KF)	≤ 0.5% w/w
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.