Description

6-n-Biotinylaminohexyl Isopropyl Phosphorofluoridate, Hemihydrate is a high-purity, biotinylated organophosphorus compound primarily used as a potent and selective active-site-directed serine hydrolase inhibitor. Its core value lies in enabling advanced biochemical research and proteomic studies through irreversible enzyme labeling, facilitating the identification and characterization of enzyme targets. This specialized reagent is essential for researchers and developers in pharmaceutical discovery, chemical biology, and diagnostic assay development who require precise molecular tools for studying enzyme function and inhibition mechanisms.

Application

• Proteomics Research: Irreversible active-site probe for labeling and identifying serine hydrolases in activity-based protein profiling (ABPP).
• Enzyme Inhibitor Development: Key intermediate or reference standard in the design and screening of novel serine protease inhibitors.
• Diagnostic Reagent Synthesis: Building block for creating biotinylated detection probes used in ELISA, blotting, and other affinity-based assays.
• Chemical Biology Tool: Used to study enzyme kinetics, mechanism of action, and target engagement in cellular and biochemical pathways.
• Pharmaceutical R&D: Employed in target validation and lead optimization stages for diseases involving dysregulated serine hydrolase activity.
• Affinity Purification: Facilitates the isolation and pull-down of specific enzyme targets from complex biological mixtures via streptavidin-biotin interaction.

Basic Information

Product Name	6-n-Biotinylaminohexyl Isopropyl Phosphorofluoridate, Hemihydrate
CAS No.	353754-93-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Biotinylated Fluorophosphonate; Biotin-FP; 6-(Biotinamido)hexyl isopropyl phosphorofluoridate hemihydrate; Activity-Based Probe for Serine Hydrolases; Biotin-Hexyl-FP; Isopropyl Phosphorofluoridate Biotin Conjugate; Biotin-Ahx-FP
EINECS	Contact for details

Quality Control

Our 6-n-Biotinylaminohexyl Isopropyl Phosphorofluoridate, Hemihydrate is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectroscopic identification (NMR, MS). A detailed Certificate of Analysis (COA) providing full specifications and test results is supplied with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. Due to its hygroscopic and moisture-sensitive nature, the container should be allowed to equilibrate to room temperature before opening to prevent condensation. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.