Description

Tubulysin E is a highly potent, naturally derived microtubule-depolymerizing cytotoxic peptide isolated from myxobacterial strains. It serves as a critical pharmacological tool and lead compound in oncology research due to its sub-nanomolar antiproliferative activity against multidrug-resistant cancer cell lines. Researchers in academic institutions, biopharmaceutical R&D labs, and contract research organizations (CROs) rely on Tubulysin E CAS NO 309935-58-8 for mechanistic studies of mitotic disruption, antibody–drug conjugate (ADC) payload development, and structure–activity relationship (SAR) optimization.

Application

• Antibody–drug conjugate (ADC) payload development for targeted cancer therapeutics
• In vitro cytotoxicity screening in human tumor cell line panels (e.g., NCI-60)
• Mechanistic studies of microtubule dynamics and mitotic spindle inhibition
• Reference standard for HPLC and LC-MS/MS method validation in bioanalytical laboratories
• Scaffold for semi-synthetic analog generation to improve pharmacokinetic properties
• Positive control in high-content phenotypic assays assessing antimitotic activity
• Tool compound for investigating resistance mechanisms to tubulin-targeting agents
• Calibration material in quantitative mass spectrometry-based proteomics workflows

Basic Information

Product Name	Tubulysin E
CAS No.	309935-58-8
Molecular Formula	C52H78N10O14
Molecular Weight	1067.24 g/mol
Synonyms	Tubulysin E; Tub-E; TUB-E; NSC 742527; UNII-7Z4WQ7X9VH; 7Z4WQ7X9VH; (3R,4S,5S,6R,7R,10R,11R,12R,13R,14R,15R,16R,17R,18R,19R,20R,21R,22R,23R,24R,25R,26R,27R,28R,29R,30R,31R,32R,33R,34R,35R,36R,37R,38R,39R,40R,41R,42R,43R,44R,45R,46R,47R,48R,49R,50R,51R,52R)-52-[(2R)-2-[(2S,3R)-3-[(2S)-2-[(2S,3R)-3-[(2S)-2-[(2S,3R)-3-[(2S)-2-amino-3-methylbutanoyl]amino]-2-methylpropanoyl]amino]-3-methylbutanoyl]amino]-3-methylpentanoyl]amino]-2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52-hexapentaconta-3,5,7,9,11,13,15,17,19,21,23,25,27,29,31,33,35,37,39,41,43,45,47,49-pentacontaene-1,2-diol; Tubulysin E (natural)
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. Each batch of Tubulysin E is verified for identity (HPLC retention time match, HRMS), assay (≥95.0% by HPLC), and impurity profile (total impurities ≤5.0%, single unknown ≤0.5%). Testing adheres to ICH Q2(R2) guidelines, and manufacturing follows ISO 9001-certified quality management practices.

Storage

Preserve in a tightly closed container, protected from light. Store at –20 °C under inert atmosphere (argon or nitrogen) to prevent degradation. Due to its hygroscopic nature, minimize exposure to ambient humidity; allow vial to equilibrate to room temperature in a desiccator before opening. Avoid repeated freeze–thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Retention time matches reference standard
Identification (HRMS)	[M+H]+ m/z 1068.2472 ± 0.005 (C52H79N10O14)
Assay (HPLC)	≥95.0%
Total Impurities	≤5.0%
Related Substances	Each ≤0.5%
Residual Solvents	Meets ICH Q3C limits
Water Content (KF)	≤5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.