Description

Hpi-4, Hedgehog Pathway Inhibitor 4 is a potent and selective small molecule inhibitor of the Hedgehog (Hh) signaling pathway, a critical regulator of cell growth and differentiation. This compound is essential for researchers and developers in the life sciences sector, providing a reliable tool for investigating developmental biology, cancer mechanisms, and potential therapeutic targets. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and biotechnology companies focused on oncology and regenerative medicine.

Application

• Biomedical Research: A key reagent for fundamental studies of the Hedgehog signaling pathway's role in embryonic development and tissue homeostasis.
• Oncology Research: Used to inhibit Hh pathway activity in various cancer cell lines (e.g., basal cell carcinoma, medulloblastoma, pancreatic cancer) to study tumor proliferation and metastasis.
• Drug Discovery & Screening: Serves as a reference compound or positive control in high-throughput screening assays to identify novel Hh pathway modulators.
• Mechanistic Studies: Employed to elucidate downstream effects and crosstalk of the Hh pathway with other signaling networks like Wnt and Notch.
• Stem Cell Research: Applied in studies investigating the role of Hh signaling in stem cell maintenance, differentiation, and organoid culture systems.
• Preclinical Development: Used in animal model studies to validate the Hh pathway as a therapeutic target and assess the efficacy of treatment strategies.

Basic Information

Product Name	Hpi-4, Hedgehog Pathway Inhibitor 4
CAS No.	302803-72-1
Molecular Formula	C₂₁H₂₀N₂O₃
Molecular Weight	348.40 g/mol
Synonyms	Hh Inhibitor IV; HhAntag-IV; Hedgehog Antagonist IV; 5-[(E)-2-(1H-Indol-3-yl)vinyl]-1,3-benzodioxole; 5-[(E)-2-(1H-Indol-3-yl)ethenyl]-1,3-benzodioxole; HPI-4; HPI 4; Hh pathway inhibitor 4
EINECS	Contact for details

Quality Control

Our Hpi-4 is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity assessment and NMR/LC-MS for structural confirmation. A detailed Certificate of Analysis (COA) documenting identity, purity, and impurity profiles is provided with every shipment to support your research and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. The product is hygroscopic (moisture-sensitive) and should be handled in a dry environment. Allow the vial to warm to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.