Description

n-Acetylalanyl-Alanyl-Prolyl-Alanine Chloromethyl Ketone is a specialized synthetic peptide derivative designed as an irreversible active-site-directed inhibitor. This compound is critical for researchers and developers requiring precise, covalent modification of serine protease enzymes. It is primarily utilized in biochemical research, pharmaceutical development, and proteomics for studying enzyme mechanisms, inhibitor design, and pathway analysis.

Application

• Biochemical Research: Used as a potent, irreversible inhibitor for mechanistic studies of serine proteases, particularly those recognizing the Ala-Pro-Ala sequence.
• Pharmaceutical Development: Serves as a key intermediate or tool compound in the design and screening of novel therapeutic protease inhibitors.
• Proteomics & Enzymology: Employed to selectively label, inactivate, or affinity-purify specific proteases from complex biological mixtures.
• Diagnostic Reagent Formulation: Can be utilized in the development of diagnostic kits and assays that measure or control specific protease activity.
• Academic & Industrial R&D: A fundamental tool in university and corporate laboratories for investigating protein function, signal transduction, and disease mechanisms.

Basic Information

Product Name	n-Acetylalanyl-Alanyl-Prolyl-Alanine Chloromethyl Ketone
CAS No.	38104-40-4
Molecular Formula	C19H30N4O6Cl
Molecular Weight	445.92 g/mol
Synonyms	Ac-AAP-A-CH2Cl; Ac-Ala-Ala-Pro-Ala-CH2Cl; N-Acetyl-Ala-Ala-Pro-Ala-chloromethyl ketone; AAPCK; Protease Inhibitor AAPCK; Ala-Ala-Pro-Ala Chloromethyl Ketone, N-Acetyl-; (S)-1-((S)-1-((S)-1-((S)-4-Chloro-3-oxo-1-(2-oxo-2-(pyrrolidin-2-yl)ethylamino)butan-2-ylamino)-1-oxopropan-2-ylamino)-3-methyl-1-oxobutan-2-ylamino)-3-methyl-1-oxobutan-2-yl acetate
EINECS	Contact for details

Quality Control

Our n-Acetylalanyl-Alanyl-Prolyl-Alanine Chloromethyl Ketone is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, MS for identity confirmation, and chiral analysis to ensure stereochemical integrity. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee compliance with your research specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Assay	≥ 97%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.