Description

Barstar CAS NO 37328-61-3 is a small, specific protein inhibitor of the ribonuclease barnase, forming an extremely tight and stable complex. This interaction is a classic model system for studying protein-protein interactions, folding, and molecular recognition in biochemistry and biophysics. It is an essential reagent for researchers in structural biology, enzymology, and protein engineering. Key industrial and academic applications include its use in biosensor development, affinity purification systems, and as a component in controlled cellular expression technologies.

Application

• Biochemical Research: Serves as a fundamental model for studying ultra-high-affinity protein-protein interactions, enzyme inhibition kinetics, and protein folding pathways.
• Biosensor Development: Utilized in the design of sensitive detection platforms where the barnase-barstar interaction provides a specific and reversible binding event.
• Affinity Purification Systems: Employed in tandem with barnase-tagged proteins for the highly specific capture and purification of target biomolecules.
• Controlled Gene Expression: Acts as a component in advanced molecular biology toolkits, such as the barnase-barstar "guard" system in plant biotechnology or inducible expression vectors.
• Structural Biology: Provides a well-characterized complex for X-ray crystallography and NMR spectroscopy studies to elucidate molecular binding interfaces.
• Diagnostic Assays: Incorporated into immunoassay formats as a non-antibody binding pair to reduce background and increase specificity.

Basic Information

Product Name	Barstar
CAS No.	37328-61-3
Molecular Formula	C284H456N84O86S2
Molecular Weight	Approx. 6520 Da
Synonyms	Barstar Protein; Barnase Inhibitor; Ribonuclease Inhibitor Barstar; BARS_HUMAN (referring to human barstar-like protein); PSTI (Pancreatic Secretory Trypsin Inhibitor) - *Note: This is a functional analog, not identical*; Barnase Barstar Complex Component; B. amyloliquefaciens Ribonuclease Inhibitor
EINECS	Contact for details

Quality Control

Our Barstar is produced and tested under stringent conditions to ensure high purity and consistent biological activity, suitable for research and industrial applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional activity. We adhere to relevant quality management standards to guarantee reliable supply and product integrity. Certificates of Analysis (COA) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C or below. Reconstituted solutions should be aliquoted and stored at -80°C to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed in a dry environment after each use.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Single band corresponding to ~10 kDa
Purity (HPLC)	≥ 95%
Protein Content (UV Absorbance)	≥ 90%
Biological Activity (Inhibition Assay)	Fully active against barnase
Endotoxin Level (LAL)	< 1.0 EU/μg
Solubility	Soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.