Description

(3S,5S)-3'-Hydroxycotinine is a major metabolite of nicotine, characterized by its specific stereochemistry. This compound is of significant value as a high-purity reference standard and analytical reagent for research and diagnostic applications. It is primarily needed by laboratories and manufacturers in the pharmaceutical, clinical diagnostics, and forensic toxicology sectors for method development, calibration, and quality control.

Application

• Pharmaceutical Reference Standard: Used as a certified reference material (CRM) for the quantification of nicotine metabolites in pharmacokinetic and metabolic studies.
• Clinical Diagnostics: Essential for calibrating assays in liquid chromatography-mass spectrometry (LC-MS/MS) systems to accurately measure nicotine exposure and metabolism in biological samples.
• Forensic Toxicology: Serves as a key analytical standard in forensic labs for confirming and quantifying nicotine and its metabolites in legal and workplace testing.
• Biochemical Research: Utilized in vitro and in vivo studies to investigate the pharmacological activity, distribution, and elimination pathways of nicotine derivatives.
• Diagnostic Kit Manufacturing: Incorporated into the production of ELISA kits and other immunoassays designed for nicotine metabolite detection.
• Method Development & Validation: Critical for developing, optimizing, and validating new analytical methods in compliance with guidelines like ICH Q2(R1).

Basic Information

Product Name	(3S,5S)-3'-Hydroxycotinine
CAS No.	37096-14-3
Molecular Formula	C10H12N2O2
Molecular Weight	192.22 g/mol
Synonyms	(3S,5S)-3-Hydroxycotinine; trans-3'-Hydroxycotinine; 5-(3-Hydroxy-1-methyl-2-pyrrolidinyl)-2-pyridinol; 3-Hydroxycotinine; Cotinine Metabolite; Nicotine Metabolite 3; 1-Methyl-5-(3-pyridyl)-2-pyrrolidinone-3-ol
EINECS	Contact for details

Quality Control

Our (3S,5S)-3'-Hydroxycotinine is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and chiral purity confirmation, to ensure it meets the high standards required for reference materials. Certificates of Analysis (COA) with detailed chromatographic data are provided for traceability and compliance.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a controlled environment to minimize exposure to atmospheric humidity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥98.0%
Chiral Purity (Chiral HPLC)	≥99.0% (3S,5S) isomer
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.