Description

Z-Gly-Gly-Phe-Chloromethylketone is a specialized peptide-based chloromethyl ketone inhibitor, a crucial tool compound in biochemical and pharmacological research. Its primary value lies in its potent and irreversible inhibition of serine proteases, particularly chymotrypsin and chymotrypsin-like enzymes, by covalently modifying the active site. This compound is essential for researchers and developers in pharmaceutical discovery, proteomics, and enzymology who require precise mechanism-based inhibitors to study enzyme function, develop therapeutic agents, or control proteolytic activity in experimental systems.

Application

• Protease Inhibition Studies: As a potent, irreversible inhibitor for characterizing the activity and specificity of chymotrypsin and related serine proteases.
• Pharmaceutical Research: A key biochemical tool in the development and screening of novel anti-inflammatory or anticancer drugs that target specific protease pathways.
• Biochemical Assay Development: Used to create controlled experimental conditions by selectively inhibiting target proteases in complex biological mixtures.
• Enzyme Mechanism Analysis: Employed in structural and kinetic studies to elucidate the catalytic mechanisms and substrate binding sites of proteolytic enzymes.
• Cell Biology & Proteomics: Useful for probing the role of specific proteases in cellular processes, signaling pathways, and protein degradation.

Basic Information

Product Name	Z-Gly-Gly-Phe-Chloromethylketone
CAS No.	35172-59-9
Molecular Formula	C22H24ClN3O6
Molecular Weight	461.90 g/mol
Synonyms	Z-Gly-Gly-Phe-CH2Cl; Benzyloxycarbonyl-Glycyl-Glycyl-L-Phenylalanine Chloromethyl Ketone; Z-Gly-Gly-Phe-CMK; N-Carbobenzoxy-Glycyl-Glycyl-L-Phenylalanine Chloromethyl Ketone; Chymotrypsin Inhibitor I; Protease Inhibitor (Chymotrypsin); CBZ-Gly-Gly-Phe-CH2Cl; Nα-CBZ-Gly-Gly-Phe-chloromethylketone
EINECS	Contact for details

Quality Control

Our Z-Gly-Gly-Phe-Chloromethylketone is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity assessment and structural confirmation via spectroscopic methods. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and potency. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.