Description

n-Methylphalloidin is a chemically modified derivative of the potent bicyclic heptapeptide phallotoxin, phalloidin. This compound is of significant value in life science research as a high-affinity molecular probe for selectively labeling filamentous actin (F-actin) in fixed cells. It is primarily utilized by researchers in cell biology, pharmacology, and toxicology for advanced fluorescence microscopy and cytoskeletal studies, enabling detailed visualization of cellular architecture and dynamics.

Application

• Fluorescent Cytoskeleton Staining: Conjugated to fluorophores (e.g., Alexa Fluor, FITC, TRITC) for high-resolution imaging of F-actin networks in fixed cell preparations.
• Biochemical Research: Used as a specific tool to study actin polymerization, depolymerization, and the mechanisms of actin-binding proteins.
• Toxicology Studies: Serves as a reference compound and intermediate in research investigating the mechanism of action of amatoxins and phallotoxins.
• Diagnostic Probe Development: Employed in the design and development of diagnostic assays and kits for cellular pathology.
• Pharmaceutical Research: Utilized in target validation and screening assays related to cytoskeletal-targeting drug discovery.

Basic Information

Product Name	n-Methylphalloidin
CAS No.	35167-13-6
Molecular Formula	C37H51N7O11S
Molecular Weight	801.91 g/mol
Synonyms	N-Methylphalloidin; N-Methylphalloidine; NSC 334719; 7β-Hydroxy-1,2,4a,6,7,7a,8,10,11,11a-decahydro-7a-hydroxy-1,8-dimethyl-13-(1-methyl-2-oxo-4-L-threonine-5-L-hydroxyproline-6-L-tryptophan-3-L-cysteine-2-L-alanine-1-γ,δ-dihydroxy-L-valine-7-L-alanine-1-aza-2-oxobicyclo[3.3.0]oct-3-yl)-5H-10,11a-epoxyisobenzofuro[3,2-f]isochromene-5,9,14-trione
EINECS	Contact for details

Quality Control

Our n-Methylphalloidin is produced and handled under strict quality management protocols to ensure batch-to-batch consistency and reliability for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and mass spectrometry for identity confirmation. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all relevant specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be kept under desiccant in a dry environment. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in DMSO, Methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.