Description

H-Phe-Cmk·HCl is a chloromethyl ketone derivative of phenylalanine, a highly specific and irreversible serine protease inhibitor. This compound is a critical research tool for studying enzyme mechanisms and regulating proteolytic activity in biological systems. It is primarily utilized by researchers and manufacturers in the pharmaceutical and life science sectors for biochemical research and drug discovery applications.

Application

• Biochemical Research: Acts as a potent and irreversible inhibitor for studying serine proteases like chymotrypsin, trypsin, and related enzymes.
• Protease Mechanism Studies: Used to elucidate enzyme active site chemistry and catalytic pathways due to its affinity-labeling properties.
• Cell Biology & Signal Transduction: Employed to inhibit specific proteases in cellular assays to understand their role in processes like apoptosis and inflammation.
• Pharmaceutical Development: Serves as a key intermediate or reference standard in the development of protease-targeted therapeutic agents.
• Diagnostic Reagent Formulation: Used in the development of diagnostic kits and assays that require controlled protease activity.

Basic Information

Product Name	H-Phe-Cmk·HCl
CAS No.	34351-19-4
Molecular Formula	C12H15Cl2NO2
Molecular Weight	276.16 g/mol
Synonyms	L-Phenylalanine Chloromethyl Ketone Hydrochloride; N-[(Benzyloxy)carbonyl]-L-phenylalanyl Chloromethane Hydrochloride; Z-Phe-CH2Cl·HCl; Phenylalanine Chloromethyl Ketone Hydrochloride; Protease Inhibitor Phe-CMK; Chymotrypsin Inhibitor; N-CBZ-L-Phenylalanine Chloromethyl Ketone Hydrochloride
EINECS	Contact for details

Quality Control

Our H-Phe-Cmk·HCl is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and spectroscopic identification (NMR, IR, MS), to ensure it meets the high standards required for research and development. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.