Description

Puromycin Dihydrochloride is a potent aminonucleoside antibiotic widely used as a critical selection agent in molecular biology and cell culture research. Its primary value lies in its ability to rapidly and irreversibly inhibit protein synthesis in eukaryotic cells, making it an indispensable tool for stable cell line generation and selection. This high-purity compound is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and life sciences industries, particularly for applications involving genetic engineering and mammalian cell culture systems.

Application

• Selection Agent in Genetic Engineering: Used for the selection of mammalian cell lines stably transfected with plasmids containing puromycin resistance genes (pac).
• Cell Culture Research: Employed to eliminate non-transfected cells and maintain selective pressure on engineered cell populations.
• Biopharmaceutical Production: Critical in the development and maintenance of producer cell lines for recombinant protein and monoclonal antibody manufacturing.
• Antibiotic Mode-of-Action Studies: Serves as a model compound for studying protein synthesis inhibition and ribosomal function.
• Virology Research: Utilized in the selection of packaging cell lines for lentiviral and retroviral vector production.
• Drug Screening Assays: Acts as a positive control in assays designed to identify novel antimicrobial or anticancer agents targeting translation.

Basic Information

Product Name	Puromycin Dihydrochloride
CAS No.	3506-23-8
Molecular Formula	C22H29N7O5 • 2HCl
Molecular Weight	544.43 g/mol
Synonyms	Puromycin 2HCl; CL 16536; NSC 3055; Stylomycin; 3'-Deoxy-N,N-dimethyl-3'-(O-methyl-L-tyrosinamido)adenosine Dihydrochloride; 6-Dimethylamino-9-[3-deoxy-3-(p-methoxy-L-phenylalanylamino)-β-D-ribofuranosyl]purine Dihydrochloride; (S)-2-Amino-N-[(2S,3S,4R,5R)-5-(6-amino-2-dimethylaminopurin-9-yl)-3,4-dihydroxy-tetrahydrofuran-2-yl]-3-(4-methoxyphenyl)propionamide Dihydrochloride
EINECS	222-506-7

Quality Control

Our Puromycin Dihydrochloride is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and NMR, and stringent controls for moisture and residual solvents. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability and potency. For long-term storage, desiccants are recommended.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time corresponds to reference standard
Assay (HPLC)	≥98.0%
Water Content (KF)	≤5.0%
pH (1% Solution)	3.0 - 5.0
Optical Rotation	-8.0° to -12.0° (c=1 in H2O)
Biological Activity	Conforms (Cell-based assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.