Description

Hemicholinium-3 is a potent and selective competitive inhibitor of the high-affinity choline uptake (HACU) system. This compound is a critical research tool for studying cholinergic neurotransmission, synaptic function, and related neurological pathways. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and biotechnology companies focused on neuroscience, neuropharmacology, and the development of therapeutic agents for cholinergic system disorders.

Application

• Neuroscience Research: A fundamental tool for investigating the synthesis, storage, and release of acetylcholine in both central and peripheral nervous systems.
• Pharmacological Studies: Used to create experimental models of cholinergic hypofunction to evaluate potential drug candidates for Alzheimer's disease, myasthenia gravis, and other cognitive disorders.
• Neurochemical Mechanism Elucidation: Employed to study the role of cholinergic transmission in learning, memory, motor control, and autonomic functions.
• In Vitro & Ex Vivo Assays: Applied in cell culture systems, brain slice preparations, and synaptosome studies to block choline transport and measure downstream effects.
• Toxicology Research: Serves as a reference compound for studying the effects of cholinergic system disruption.
• Biochemical Pathway Analysis: Used to dissect and validate specific steps within the cholinergic metabolic pathway.

Basic Information

Product Name	Hemicholinium-3
CAS No.	312-45-8
Molecular Formula	C₂₄H₃₂Br₂N₂O₂
Molecular Weight	540.34 g/mol
Synonyms	Hemicholinium-3 bromide; HC-3; 2,2'-(4,4'-Biphenylylene)bis(2-hydroxy-4,4-dimethylmorpholinium) dibromide; 4,4'-Biphenylenebis(2-hydroxy-4,4-dimethylmorpholinium) dibromide; 1,1'-(1,4-Phenylenebis(methylene))bis(2-hydroxy-4,4-dimethylmorpholinium) dibromide; NSC 169061; Choline uptake inhibitor HC-3
EINECS	206-228-9

Quality Control

Our Hemicholinium-3 is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot undergoes comprehensive analytical testing, including identity confirmation, purity assay, and impurity profiling. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting compliance with our stringent in-house specifications. We support research requiring traceable, high-quality biochemicals.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and must be kept in a desiccated environment to maintain stability and purity. The container should be kept tightly sealed when not in use.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.