Description

Bacterial Sortase Substrate Iii, Abz/Dnp is a specialized fluorogenic peptide substrate designed for the study of sortase enzymes, which are critical for bacterial cell wall assembly and virulence. This compound enables high-throughput screening and precise kinetic analysis of sortase activity, a key target in antibacterial drug discovery and protein engineering. It is an essential research tool for pharmaceutical R&D, academic biochemistry, and biotechnology companies focused on developing novel anti-infective therapies and bio-conjugation strategies.

Application

• Sortase Enzyme Activity Assays: Primary substrate for continuous fluorometric monitoring of sortase A and other sortase family enzymes.
• High-Throughput Screening (HTS): Enables rapid identification and characterization of sortase inhibitors or activators in drug discovery campaigns.
• Biochemical Research: Used in fundamental studies of bacterial pathogenesis, cell wall anchoring mechanisms, and transpeptidation reactions.
• Method Development & Validation: Serves as a standard for developing and optimizing new assay protocols for sortase activity.
• Protein Labeling & Conjugation Studies: A model substrate for exploring sortase-mediated ligation (SML) techniques in protein engineering and bioconjugation.
• Quality Control in Enzyme Production: Used to assess the specific activity and purity of commercially produced sortase enzymes.

Basic Information

Product Name	Bacterial Sortase Substrate Iii, Abz/Dnp
CAS No.	2857862-12-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Abz-LPETG-Dnp; Sortase Substrate III (Abz/Dnp); 2-Aminobenzoyl-Leu-Pro-Glu-Thr-Gly-2,4-Dinitrophenyl; Fluorogenic Sortase A Substrate; LPETG-based fluorogenic peptide; Sortase transpeptidation substrate; SrtA substrate Abz-LPETG-Dnp
EINECS	Contact for details

Quality Control

Our Bacterial Sortase Substrate Iii, Abz/Dnp is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive biochemical assays. Each lot is characterized by advanced analytical techniques including HPLC for purity and MS for identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, assay results, and functional activity data is provided with every shipment to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Functional Activity	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.