Description

Ezh2-In-2 CAS NO 2238821-31-1 is a potent, selective, and cell-active small molecule inhibitor of the Enhancer of Zeste Homolog 2 (EZH2) methyltransferase. This compound matters for researchers as it provides a critical tool for modulating histone methylation and studying epigenetic regulation in disease models. It is primarily needed by pharmaceutical R&D teams and academic laboratories focused on oncology, epigenetics, and the development of novel therapeutics targeting polycomb repressive complex 2 (PRC2).

Application

• Biochemical Research: Used as a reference standard and tool compound for in vitro enzymatic assays to study EZH2 inhibition kinetics and specificity.
• Cell-Based Studies: Applied in cellular models to investigate the role of H3K27me3 in gene silencing, cell differentiation, proliferation, and apoptosis.
• Oncology Research: A key reagent for exploring the therapeutic potential of EZH2 inhibition in various cancers, including lymphomas, glioblastomas, and other solid tumors.
• Epigenetics Screening: Serves as a positive control in high-throughput screening campaigns to identify new epigenetic modulators.
• Mechanism of Action Studies: Utilized to validate target engagement and downstream effects in proof-of-concept studies for drug discovery programs.
• Preclinical Development: Supports early-stage in vivo pharmacokinetic and pharmacodynamic studies in animal models of disease.

Basic Information

Item	Detail
Product Name	Ezh2-In-2
CAS No.	2238821-31-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	EZH2-IN-2; EZH2 Inhibitor 2; UNC1999 analog; Enhancer of Zeste Homolog 2 Inhibitor 2; (S)-Pyridone-based EZH2 inhibitor; PRC2 inhibitor; (S)-1-(sec-Butyl)-N-((4,6-dimethyl-2-oxo-1,2-dihydropyridin-3-yl)methyl)-3-methyl-6-(6-(piperazin-1-yl)pyridin-3-yl)-1H-indazole-4-carboxamide
EINECS	Contact for details

Quality Control

Our Ezh2-In-2 is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for critical research. Each lot undergoes comprehensive analytical characterization, including HPLC for purity assessment and LC-MS for identity confirmation, to meet the high standards required for biochemical and cellular applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and other relevant parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This product is hygroscopic (moisture-sensitive) and should be handled under dry conditions to ensure long-term stability. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum conforms to structure
Purity (HPLC)	≥95%
Water Content (KF)	≤1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.