Description

n-(m-Peg4)-N'-(Biotin-Peg2-Amido-Peg4)-Cy5 is a sophisticated, multi-functional bioconjugation reagent designed for advanced life science research and diagnostic applications. This compound integrates a near-infrared cyanine dye (Cy5) with biotin and polyethylene glycol (PEG) linkers, enabling highly specific labeling, detection, and purification of biomolecules. It is essential for researchers and developers in pharmaceutical R&D, in vitro diagnostics (IVD), and proteomics who require precise, stable, and low-background signal generation.

Application

• Biomolecule Labeling & Conjugation: Covalent attachment of the Cy5 fluorophore and biotin tag to antibodies, proteins, peptides, and oligonucleotides for dual-mode detection.
• In Vitro Diagnostics (IVD): Development of fluorescent immunoassays (e.g., ELISA, lateral flow assays) and biosensors utilizing the streptavidin-biotin interaction for signal amplification.
• Live-Cell & In Vivo Imaging: Labeling of targeting ligands (e.g., antibodies, peptides) for near-infrared fluorescence (NIRF) imaging due to the deep tissue penetration of Cy5.
• Pull-Down Assays & Affinity Purification: Immobilization of bait molecules for the isolation and analysis of protein complexes or other interacting partners.
• Flow Cytometry & Microscopy: Creation of high-performance fluorescent probes for cell surface marker staining and intracellular target visualization.
• Drug Delivery & Targeting Research: Conjugation to nanoparticles or therapeutic agents to study biodistribution, targeting efficiency, and cellular uptake pathways.

Basic Information

Product Name	n-(m-Peg4)-N'-(Biotin-Peg2-Amido-Peg4)-Cy5
CAS No.	2107273-78-7
Molecular Formula	C84H128N10O24S2
Molecular Weight	~1702.1 g/mol
Synonyms	Biotin-PEG2-amido-PEG4-Cy5 maleimide; Cy5 Maleimide Biotin-PEG2-PEG4 Conjugate; Biotin-PEG2-PEG4-Cy5 Maleimide; N-(3-Maleimidopropionyl)-N'-(Biotin-PEG2-amido-PEG4)-Cy5; Biotin-PEG6-Cy5 Maleimide (common descriptor); Biotinylated Cy5 Maleimide PEG Linker; 2107273-78-7; Biotin-PEG2-PEG4-Cy5-Mal
EINECS	Contact for details

Quality Control

Every batch of n-(m-Peg4)-N'-(Biotin-Peg2-Amido-Peg4)-Cy5 is manufactured and analyzed under strict quality protocols to ensure high purity, consistent performance, and batch-to-batch reproducibility. Our products undergo rigorous quality testing, including HPLC, NMR, and mass spectrometry. Certificates of Analysis (COA) detailing purity, identity, and functional performance are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	Dark blue to purple solid or film
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with theoretical molecular weight
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Activity	Confirmed by conjugation and binding assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.