Description

Propargyl-Peg6-Nhs Ester is a heterobifunctional polyethylene glycol (PEG) linker featuring a terminal alkyne (propargyl) group and an N-hydroxysuccinimide (NHS) ester. This compound is specifically engineered for the efficient and site-specific conjugation of biomolecules, enabling the creation of stable amide bonds under mild aqueous conditions. It is an essential reagent for researchers and developers in the fields of bioconjugation, proteomics, and advanced drug delivery systems, particularly for click chemistry applications and PEGylation strategies.

Application

• Bioconjugation and Protein Labeling: The NHS ester reacts with primary amines on proteins, peptides, or antibodies, while the propargyl group enables subsequent copper-catalyzed azide-alkyne cycloaddition (CuAAC) for attaching fluorescent dyes, drugs, or other functional probes.
• PEGylation of Therapeutics: Used to attach PEG chains to drug molecules, peptides, or proteins to enhance solubility, improve pharmacokinetics, reduce immunogenicity, and prolong circulation half-life.
• Surface Functionalization: Modification of nanoparticles, biosensors, or microarray surfaces with alkyne groups for the immobilization of azide-containing biomolecules via click chemistry.
• Synthesis of ADC Linkers: Serves as a key intermediate in the design and synthesis of antibody-drug conjugate (ADC) linkers, providing a hydrophilic PEG spacer and a bioorthogonal handle for drug attachment.
• Crosslinking and Material Science: Building block for creating hydrogels, polymer networks, and functionalized materials with tailored biological activity and controlled release properties.
• Diagnostic Reagent Development: Facilitating the creation of sensitive and specific diagnostic assays by conjugating detection molecules to targeting agents.

Basic Information

Product Name	Propargyl-Peg6-Nhs Ester
CAS No.	2093153-99-0
Molecular Formula	C28H47NO13
Molecular Weight	605.68 g/mol
Synonyms	Propargyl-PEG6-NHS ester; Propargyl-PEG6-NHS ester; NHS-PEG6-Propargyl; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-[2-[2-[2-[2-(2-prop-2-ynoxyethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]propanoate; Propargyl-O-(CH2CH2O)6-CH2CH2COO-NHS; Alkyne-PEG6-NHS ester; Propargyl PEG6 NHS Ester; Propargyl-PEG6-NHS
EINECS	Contact for details

Quality Control

Our Propargyl-Peg6-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment, NMR for structural confirmation, and MS for identity verification, to ensure it meets the high standards required for sensitive research and development applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous liquid
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.