Description

n-Boc-n-Bis(Peg3-Nhs Ester) is a specialized heterobifunctional crosslinking reagent featuring a Boc-protected amine and two NHS-activated PEG3 ester groups. This compound is engineered to facilitate the controlled conjugation of biomolecules, offering extended solubility and reduced steric hindrance due to its polyethylene glycol (PEG) spacer arms. It is an essential tool for researchers and manufacturers in bioconjugation, pharmaceutical development, and advanced material science, enabling the creation of stable, well-defined molecular constructs.

Application

• Bioconjugation and Protein Modification: For site-specific labeling of antibodies, enzymes, and other proteins with fluorescent dyes, drugs, or other functional molecules.
• ADC (Antibody-Drug Conjugate) Development: As a key linker-payload component to attach cytotoxic agents to monoclonal antibodies via a cleavable or non-cleavable linkage.
• Peptide and Oligonucleotide Synthesis: Used to introduce PEGylated linkers or functional handles during solid-phase or solution-phase synthesis.
• Surface Functionalization: For modifying nanoparticles, biosensors, and microarray surfaces with amine-reactive groups to immobilize biomolecules.
• Polymer and Dendrimer Chemistry: Serves as a branching or crosslinking unit in the synthesis of functionalized polymers and dendritic structures.
• Diagnostic Reagent Formulation: In the development of immunoassay components and diagnostic probes requiring stable, hydrophilic linkers.

Basic Information

Product Name	n-Boc-n-Bis(Peg3-Nhs Ester)
CAS No.	2055042-31-2
Molecular Formula	C33H56N4O16
Molecular Weight	764.82 g/mol
Synonyms	1,1'-(2,2'-(2-(2-(3-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)propanamido)ethyl)azanediyl)bis(ethane-2,1-diyl))bis(oxy))bis(ethane-2,1-diyl) diacrylate (related Fmoc precursor); Boc-NH-PEG3-NHS ester (bis); tert-Butyl (2-(2-(2-(2,5-dioxopyrrolidin-1-yl)oxy)-2-oxoethoxy)ethoxy)ethyl)(2-(2-(2-(2,5-dioxopyrrolidin-1-yl)oxy)-2-oxoethoxy)ethoxy)ethyl)carbamate; Heterobifunctional PEG crosslinker, Boc-amine, bis-NHS ester; N-Boc-N-bis(PEG3-N-hydroxysuccinimide ester); 2055042-31-2
EINECS	Contact for details

Quality Control

Our n-Boc-n-Bis(Peg3-Nhs Ester) is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the high standards required for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability, consistency, and compliance with research and development specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and the NHS ester groups are susceptible to hydrolysis. For long-term stability, store under an inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms (M+Na+ or M+H+)
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.