Description

n-(9-Fluorenylmethoxycarbonyl)-L-Valine-1-13C is a stable isotope-labeled derivative of the amino acid L-Valine, where the carbon at the 1-position is enriched with the 13C isotope. This compound is critically important for advanced research and development, providing a non-radioactive tracer for precise metabolic and pharmacokinetic studies. It is an essential building block for pharmaceutical chemists and researchers in proteomics and peptide synthesis who require high-fidelity analytical standards and labeled intermediates.

Application

• Isotope-Labeled Peptide Synthesis: Used as a 13C-labeled Fmoc-protected amino acid for the solid-phase synthesis of isotopically labeled peptides for mass spectrometry-based quantification.
• Metabolic Pathway Tracing: Serves as a crucial reagent in metabolic flux analysis and drug metabolism studies to track the incorporation of valine into proteins and other biomolecules.
• Pharmaceutical Reference Standard: Acts as a certified reference material (CRM) or internal standard in LC-MS and GC-MS assays for drug development and bioanalytical method validation.
• Proteomics Research: Enables the creation of heavy-isotope-labeled peptides for use in quantitative proteomics techniques like SILAC (Stable Isotope Labeling by Amino acids in Cell culture).
• Biochemical Research: Employed in mechanistic studies of enzyme action and protein folding where tracking specific carbon atoms is required.
• API Intermediate Development: Utilized in the research and development of novel peptide-based Active Pharmaceutical Ingredients (APIs) that require isotopic labeling.

Basic Information

Product Name	n-(9-Fluorenylmethoxycarbonyl)-L-Valine-1-13C
CAS No.	286460-74-0
Molecular Formula	C20H2013CNO4
Molecular Weight	~339.38 g/mol (for 13C labeled)
Synonyms	Fmoc-L-valine-1-13C; Fmoc-Val-1-13C; (2S)-2-[(9H-Fluoren-9-ylmethoxy)carbonylamino]-3-methyl(1-13C)butanoic acid; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-L-valine-1-13C; N-Fmoc-L-valine-1-13C; L-Valine-1-13C, N-[(9H-fluoren-9-ylmethoxy)carbonyl]-; Fmoc-13C1-L-valine
EINECS	Contact for details

Quality Control

Every batch of n-(9-Fluorenylmethoxycarbonyl)-L-Valine-1-13C is manufactured and tested under strict quality management systems. We guarantee high chemical and isotopic purity, with comprehensive testing including HPLC for chemical purity, NMR and MS for structural and isotopic confirmation, and specific optical rotation verification. Certificates of Analysis (COA) detailing all specifications, including 13C isotopic enrichment, are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and must be kept under anhydrous conditions. For long-term storage, consider storing under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
13C Isotopic Enrichment	≥ 99 atom % 13C
Specific Optical Rotation	-15.0° to -18.0° (c=1 in DMF)
Water Content (KF)	≤ 0.5%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.