Description

Cytosine-2,4-13C2-15N3 is a stable isotope-labeled analog of the fundamental nucleobase cytosine, specifically enriched with carbon-13 at the 2 and 4 positions and nitrogen-15 at all three nitrogen positions. This compound is essential for advanced analytical research, providing an internal standard for precise quantification and metabolic pathway elucidation in mass spectrometry-based studies. It is primarily utilized by researchers and manufacturers in pharmaceutical development, clinical diagnostics, and biochemistry for tracer studies, drug metabolism and pharmacokinetics (DMPK) research, and proteomics.

Application

• Internal Standard for LC-MS/MS: Serves as a critical quantitative reference standard in liquid chromatography-tandem mass spectrometry for accurate measurement of endogenous cytosine and its derivatives in complex biological matrices.
• Metabolic Flux Analysis (MFA): Used as a tracer to study the dynamics of nucleotide and nucleic acid metabolism in cell cultures, tissues, and whole organisms.
• Drug Metabolism & Pharmacokinetics (DMPK): Enables the detailed tracking of the metabolic fate of nucleoside-based pharmaceuticals and the study of their absorption, distribution, metabolism, and excretion.
• Proteomics & Biomarker Discovery: Facilitates stable isotope labeling strategies for the relative and absolute quantification of proteins and the identification of disease biomarkers.
• NMR Spectroscopy: Acts as a probe in nuclear magnetic resonance studies to investigate molecular structure, dynamics, and interactions without spectral interference from natural abundance isotopes.
• Synthesis of Labeled Nucleosides/Nucleotides: A key building block for the chemical or enzymatic synthesis of isotopically labeled cytidine, deoxycytidine, and their corresponding triphosphates for further research applications.

Basic Information

Product Name	Cytosine-2,4-13C2-15N3
CAS No.	285978-06-5
Molecular Formula	C313C2H515N3O
Molecular Weight	117.06 g/mol (for labeled form)
Synonyms	4-Amino-2(1H)-pyrimidinone-2,4-13C2-1,3,5-15N3; [2,4-13C2,1,3,5-15N3]Cytosine; 13C2,15N3-Cytosine; Cytosine-13C2,15N3; Isotopically Labeled Cytosine; 6-Amino-2-hydroxy-1,3,5-triazine-2,4-13C2-1,3,5-15N3; 2-Hydroxy-4-aminopyrimidine-2,4-13C2-1,3,5-15N3
EINECS	Contact for details

Quality Control

Our Cytosine-2,4-13C2-15N3 is manufactured under strict quality systems to ensure the highest isotopic and chemical purity for sensitive research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (Atom % 13C and 15N), chemical purity, and identity confirmation. We adhere to relevant industry standards for stable isotope-labeled compounds, ensuring reliable performance as an internal standard and tracer.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This material is hygroscopic (moisture-sensitive); keep the container tightly sealed in a dry environment.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (Atom % 13C)	≥ 99% at positions 2 & 4
Isotopic Enrichment (Atom % 15N)	≥ 98% at positions 1, 3, & 5
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.