Description

Ro 27-3225 is a selective, high-potency corticotropin-releasing factor (CRF) receptor type 1 (CRF1) antagonist developed for preclinical and pharmaceutical research. It is valued for its robust in vitro binding affinity and functional antagonism, enabling precise mechanistic studies of stress-related neuroendocrine pathways. This compound is essential for neuroscience, psychopharmacology, and drug discovery programs targeting anxiety, depression, and irritable bowel syndrome (IBS). Ro 27-3225 CAS NO 274682-89-2 is supplied as a research-grade chemical meeting stringent purity and traceability standards for global laboratory use.

Application

• Preclinical investigation of CRF1 receptor signaling in rodent models of chronic stress and anxiety-like behavior
• In vitro functional assays (cAMP inhibition, calcium flux) to characterize CRF1 antagonist potency and selectivity
• Pharmacokinetic and brain penetration studies in CNS drug development programs
• Biomarker validation in translational models linking HPA axis dysregulation to mood disorders
• Reference standard for analytical method development (HPLC, LC-MS/MS) in neuropharmacological quality control
• Tool compound for target engagement studies using radiolabeled or fluorescent CRF1 probe competition assays
• Co-administration with CRF or urocortin in electrophysiology experiments to assess synaptic modulation
• Lead optimization support in medicinal chemistry campaigns focused on non-peptidic CRF antagonists

Basic Information

Product Name	Ro 27-3225
CAS No.	274682-89-2
Molecular Formula	C27H28ClN5O3
Molecular Weight	505.99 g/mol
Synonyms	Ro-27-3225; RO273225; (R)-2-(2-Chloro-4-methoxyphenyl)-N-((1S,2R)-2-(4-fluorophenyl)cyclopropyl)-4,5-dihydro-1H-imidazole-1-carboxamide; CRF1 antagonist Ro 27-3225; Corticotropin-Releasing Factor Receptor 1 Antagonist; N-[(1S,2R)-2-(4-Fluorophenyl)cyclopropyl]-2-(2-chloro-4-methoxyphenyl)-4,5-dihydro-1H-imidazole-1-carboxamide; Ro273225; CRF-R1 antagonist
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. Each batch of Ro 27-3225 is verified for identity (HPLC retention time, IR spectroscopy), assay (≥98.5% by HPLC), residual solvents (ICH Q3C compliant), heavy metals (<10 ppm), and microbial limits (where applicable). Testing follows ACS Reagent Grade and EP/USP general chapters guidelines for research chemicals.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8°C under inert atmosphere (argon or nitrogen) to prevent degradation. Due to its hygroscopic nature, maintain in a low-humidity environment (<30% RH) with desiccant. Avoid repeated exposure to ambient air during handling.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥98.5%
Related Substances (HPLC)	Single impurity ≤0.3%; Total impurities ≤0.5%
Residual Solvents (GC)	Meets ICH Q3C Class 3 limits
Water (Karl Fischer)	≤0.5%
Heavy Metals	<10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.