Description

Acrolein 2,4-Dinitrophenylhydrazone-D3 is a deuterated derivative of the classic 2,4-dinitrophenylhydrazone (DNPH) derivative of acrolein, a key analytical reagent. This compound is specifically designed for use as an internal standard in mass spectrometry, enabling highly accurate and sensitive quantification of acrolein and related carbonyl compounds in complex matrices. It is an essential tool for analytical laboratories in the environmental monitoring, food safety, and industrial hygiene sectors, where precise measurement of trace-level aldehydes is critical for compliance and research.

Application

• Internal Standard for LC-MS/MS Analysis: Primary use as a stable isotope-labeled internal standard for the quantification of acrolein and other reactive aldehydes via derivatization with DNPH followed by liquid chromatography-tandem mass spectrometry.
• Environmental Air & Water Monitoring: Enables accurate measurement of acrolein, a hazardous air pollutant and water contaminant, in environmental samples for regulatory compliance and risk assessment studies.
• Food Safety & Quality Control: Used in the analysis of lipid oxidation products, such as acrolein, in edible oils, fried foods, and other fat-containing products to assess freshness and safety.
• Industrial Hygiene & Occupational Health: Critical for monitoring workplace exposure to acrolein in industries like chemical manufacturing, petroleum refining, and firefighting.
• Biomarker Research: Facilitates the precise measurement of acrolein-DNA or protein adducts in biological samples (e.g., blood, urine) for studies on oxidative stress, smoking-related diseases, and metabolic disorders.
• Method Development & Validation: Serves as a key reagent for developing, optimizing, and validating robust analytical methods for carbonyl compound analysis, improving accuracy and reproducibility.
• Pharmaceutical Impurity Profiling: Aids in the detection and quantification of genotoxic impurities like acrolein in active pharmaceutical ingredients (APIs) and finished drug products.
• Tobacco & E-Cigarette Research: Employed in the analysis of toxic aldehydes, including acrolein, in mainstream and sidestream smoke and in e-cigarette vapor.

Basic Information

Item	Detail
Product Name	Acrolein 2,4-Dinitrophenylhydrazone-D3
CAS No.	259824-62-9
Molecular Formula	C9H5D3N4O4
Molecular Weight	227.19 g/mol (approx.)
Synonyms	Acrolein-DNPH-D3; Acrolein-d3 2,4-Dinitrophenylhydrazone; 2-Propenal, 2,4-dinitrophenylhydrazone-d3; Acrolein (2,4-DNPH)-d3; Deuterated Acrolein DNPH Derivative; Acrolein-d3 DNPH; Acrolein Internal Standard-d3; (E)-Acrolein-2,4-dinitrophenylhydrazone-d3
EINECS	Contact for details

Quality Control

Every batch of Acrolein 2,4-Dinitrophenylhydrazone-D3 is produced and analyzed under strict quality management protocols to ensure the high chemical purity and isotopic enrichment required for reliable analytical results. Our quality commitment includes verification of identity, assay, and deuterium incorporation. A comprehensive Certificate of Analysis (COA) detailing lot-specific results for purity (by HPLC), isotopic purity (by MS), and other critical parameters is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (refrigerated) or as indicated on the label or COA. For long-term storage, keep the container in a freezer (-20°C) under an inert atmosphere. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Orange to red crystalline powder or solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Area %)
Isotopic Purity (D3)	≥ 98 atom % D
Water Content (KF)	≤ 1.0 %
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.