Description

L-Threonine-15N is a stable isotope-labeled analog of the essential amino acid L-Threonine, where the nitrogen atom is enriched with the 15N isotope. This compound is a critical tool in advanced research and analytical chemistry, enabling precise metabolic pathway tracing, protein turnover studies, and quantitative proteomics. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and manufacturers of high-value diagnostic reagents and internal standards for mass spectrometry.

Application

• Biomedical Research: As a stable isotope tracer for studying amino acid metabolism, protein synthesis rates, and nitrogen utilization in cell cultures and animal models.
• Proteomics & Metabolomics: Serving as an internal standard in Liquid Chromatography-Mass Spectrometry (LC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS) for the accurate quantification of L-Threonine in complex biological samples.
• Pharmaceutical Development: Used in the research and development of new drugs, particularly in pharmacokinetic (PK) and absorption, distribution, metabolism, and excretion (ADME) studies to track drug metabolites.
• Nutritional Science: Investigating threonine requirements, bioavailability, and metabolic fate in nutritional studies and for developing specialized clinical nutrition products.
• Isotope Labeling: As a building block for the chemical synthesis of more complex 15N-labeled peptides, proteins, or other bioactive molecules.
• Diagnostic Reagents: A key raw material in manufacturing calibration standards and quality control materials for clinical mass spectrometry assays.

Basic Information

Product Name	L-Threonine-15N
CAS No.	252743-41-2
Molecular Formula	C₄H₉¹⁵NO₃
Molecular Weight	120.10 g/mol (for ¹⁵N labeled form)
Synonyms	(2S,3R)-2-Amino-3-hydroxybutanoic acid-15N; L-(-)-Threonine-15N; (2S,3R)-2-Azanyl-3-hydroxy-butanoic acid-15N; Threonine-15N; 15N-L-Threonine; Amino acid-15N (Threonine); Stable isotope-labeled threonine
EINECS	Contact for details

Quality Control

Our L-Threonine-15N is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including isotopic purity (enrichment) by NMR or MS, chemical purity by HPLC, and optical rotation verification to ensure identity and potency. Certificates of Analysis (COA) detailing batch-specific results for assay, isotopic enrichment, and impurities are provided and available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and must be kept in a desiccated environment to maintain stability and purity. For long-term storage, consider under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Isotopic Enrichment (¹⁵N)	≥ 98 atom % ¹⁵N
Optical Rotation	-28.0° to -30.0° (c=1 in H₂O)
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.