Description

(3'R,5'S)-3'-Hydroxycotinine is a major, pharmacologically active metabolite of nicotine, characterized by its specific stereochemistry. This compound is of significant value as a high-purity reference standard and analytical reagent for research and diagnostic applications. It is primarily needed by pharmaceutical R&D laboratories, clinical toxicology facilities, and analytical service providers for method development, calibration, and metabolite quantification studies.

Application

• Pharmaceutical Reference Standard: Used for the quantification and identification of nicotine metabolites in pharmacokinetic and pharmacodynamic studies.
• Clinical Toxicology & Diagnostics: Serves as a critical calibrant in LC-MS/MS and HPLC methods for accurate measurement of nicotine exposure and metabolism in biological samples (e.g., urine, plasma).
• Biomarker Research: Employed in studies investigating the relationship between nicotine metabolism, genetic polymorphisms (e.g., CYP2A6), and smoking behavior or disease risk.
• Forensic Analysis: Utilized as a certified reference material in forensic laboratories for confirmatory testing in drug exposure cases.
• Metabolite Synthesis: Acts as a key chiral intermediate or precursor for the synthesis of more complex nicotine-related metabolites or labeled analogs.
• Quality Control: Applied in the manufacturing and QC of diagnostic kits and assays designed to detect nicotine and its metabolites.

Basic Information

Product Name	(3'R,5'S)-3'-Hydroxycotinine
CAS No.	223655-46-7
Molecular Formula	C10H12N2O2
Molecular Weight	192.22 g/mol
Synonyms	trans-3'-Hydroxycotinine; (R,S)-3'-Hydroxycotinine; 5'-Hydroxycotinine; 3-Hydroxycotinine; 1-Methyl-5-(3-pyridyl)-2-pyrrolidinone-3-ol; Nicotine metabolite; Cotinine metabolite
EINECS	Contact for details

Quality Control

Our (3'R,5'S)-3'-Hydroxycotinine is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including chiral purity verification, to ensure it meets the high standards required for research and diagnostic use. Certificates of Analysis (COA) detailing identity, purity, and impurity profiles by HPLC are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. For long-term storage, consider storing at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Chiral Purity (Chiral HPLC)	≥ 99.0% (3'R,5'S isomer)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.