Description

4-Hydroxy-17Beta-Estradiol-1,2,16,16,17-D5 is a deuterium-labeled analog of a key estrogen metabolite, designed for use as an internal standard in advanced analytical techniques. This high-purity, isotopically labeled compound is critical for ensuring the accuracy and precision of quantitative measurements in complex biological matrices. It is primarily utilized by research institutions, pharmaceutical developers, and clinical diagnostic laboratories engaged in hormone analysis, metabolic studies, and pharmacokinetic research.

Application

• Internal Standard for LC-MS/MS: Essential for the precise quantification of endogenous estradiol and its metabolites in bioanalytical methods, correcting for matrix effects and instrument variability.
• Metabolic Pathway Research: Used as a tracer to study the formation, distribution, and clearance of 4-hydroxyestradiol in in vitro and in vivo metabolic studies.
• Clinical Diagnostics Development: Employed in the development and validation of high-sensitivity assays for hormonal profiling in endocrinology and oncology.
• Pharmaceutical R&D: Supports drug metabolism and pharmacokinetics (DMPK) studies for novel therapeutics targeting estrogen pathways.
• Forensic Toxicology: Aids in the accurate detection and confirmation of steroid hormones in forensic analyses.
• Reference Material: Serves as a certified reference material (CRM) for calibrating analytical instruments and ensuring method compliance.

Basic Information

Item	Detail
Product Name	4-Hydroxy-17Beta-Estradiol-1,2,16,16,17-D5
CAS No.	221093-38-5
Molecular Formula	C18H19D5O3
Molecular Weight	~279.4 g/mol
Synonyms	4-Hydroxyestradiol-d5; 1,2,16,16,17-D5-4-Hydroxy-17β-estradiol; 4,17β-Dihydroxyestra-1,3,5(10)-trien-3-ol-1,2,16,16,17-d5; Estra-1,3,5(10)-triene-3,4,17β-triol-1,2,16,16,17-d5; Deuterated 4-Hydroxyestradiol; 4-OHE2-d5; [D5]-4-Hydroxy-17β-estradiol
EINECS	Contact for details

Quality Control

Every batch of 4-Hydroxy-17Beta-Estradiol-1,2,16,16,17-D5 is manufactured under strict quality systems and undergoes comprehensive analytical characterization. We guarantee high chemical and isotopic purity to meet the exacting demands of quantitative mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting purity by HPLC, isotopic enrichment by MS, and confirming structural identity.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. The product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain integrity. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity	≥ 95%
Solubility	Soluble in DMSO, ethanol, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.