Description

Caspase-3/7 Inhibitor I is a potent, cell-permeable, and reversible inhibitor of caspase-3 and caspase-7, key executioner proteases in the apoptotic pathway. This compound is essential for researchers investigating programmed cell death mechanisms, offering a critical tool to modulate apoptosis in experimental models. It is primarily utilized by scientists in the fields of pharmaceutical R&D, biochemistry, and cell biology for target validation, pathway analysis, and the development of novel therapeutic strategies for cancer and neurodegenerative diseases.

Application

• Apoptosis Research: Fundamental tool for studying the role of caspase-3/7 in intrinsic and extrinsic apoptotic pathways.
• Drug Discovery & Screening: Used in high-throughput assays to identify and evaluate novel compounds that modulate caspase activity.
• Disease Model Studies: Applied in cellular and animal models of conditions like ischemia-reperfusion injury, neurodegenerative disorders (e.g., Alzheimer's, Parkinson's), and autoimmune diseases to inhibit apoptosis.
• Biochemical Assay Development: Serves as a positive control or reference standard in fluorometric and colorimetric caspase activity assays.
• Cell Culture Protection: Used to prevent unwanted apoptosis in primary cell cultures or sensitive cell lines during experimental manipulation.
• Mechanistic Toxicology: Helps determine if observed cell death is mediated through caspase-3/7-dependent apoptosis.

Basic Information

Product Name	Caspase-3/7 Inhibitor I
CAS No.	220509-74-0
Molecular Formula	C₂₀H₁₆N₄O₃
Molecular Weight	360.37 g/mol
Synonyms	Z-DEVD-FMK; Benzyloxycarbonyl-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-fluoromethyl ketone; Caspase-3 Inhibitor II; DEVD-FMK; (2S)-5-[[(2S)-4-[[(2S)-1-[[(2S)-2-Benzyloxycarbonylamino-3-methoxy-3-oxopropyl]amino]-3-methoxy-1,3-dioxopropan-2-yl]amino]-3-methoxy-4-oxobutanoyl]amino]-2-fluoropentanoic acid fluoromethyl ester
EINECS	Contact for details

Quality Control

Our Caspase-3/7 Inhibitor I is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for critical research. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting purity, assay results, and storage specifications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥95%
Water Content (KF)	≤1.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.