Description

Z-Ala-Pro-Phe-Chloromethylketone is a potent and selective irreversible inhibitor of serine proteases, specifically designed as a peptide-based chloromethyl ketone (CMK) inhibitor. This compound is a critical research tool for studying enzyme kinetics, active site mapping, and the physiological roles of proteases like chymotrypsin and related enzymes. It is primarily utilized by pharmaceutical researchers, biochemists, and academic institutions engaged in proteomics, drug discovery, and the development of therapeutic enzyme inhibitors.

Application

• Protease Inhibition Studies: Acts as an active-site directed irreversible inhibitor for mechanistic studies of serine proteases, particularly those with chymotrypsin-like specificity.
• Biochemical Research: Essential tool for characterizing enzyme activity, inhibition constants (Ki), and for validating assay systems in vitro.
• Drug Discovery & Development: Serves as a key pharmacophore or starting point for designing more potent and selective therapeutic protease inhibitors.
• Proteomics & Target Validation: Used to selectively inhibit target proteases in complex biological samples to elucidate their functional pathways.
• Academic & Industrial R&D: A standard reagent in laboratories focused on enzymology, peptide chemistry, and medicinal chemistry.

Basic Information

Product Name	Z-Ala-Pro-Phe-Chloromethylketone
CAS No.	217658-18-9
Molecular Formula	C26H30ClN3O6
Molecular Weight	516.0 g/mol
Synonyms	Z-Ala-Pro-Phe-CH2Cl; Z-APF-CMK; Benzyloxycarbonyl-Ala-Pro-Phe-chloromethyl ketone; N-CBZ-Ala-Pro-Phe-chloromethyl ketone; Chymostatin analog; Serine protease inhibitor; Ala-Pro-Phe-CK (Z-blocked); N-α-Carbobenzoxy-L-alanyl-L-prolyl-L-phenylalanyl chloromethane
EINECS	Contact for details

Quality Control

Our Z-Ala-Pro-Phe-Chloromethylketone is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant standards for research-grade biochemicals to guarantee reliable and reproducible performance in your experiments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.