Description

γ-Secretase Inhibitor Xxi is a potent and selective small molecule inhibitor of the γ-secretase enzyme complex. This compound is a critical research tool for investigating the Notch signaling pathway and its role in cellular differentiation, development, and disease progression. It is essential for researchers and pharmaceutical developers in neuroscience, oncology, and regenerative medicine who require high-purity biochemicals for target validation and drug discovery programs.

Application

• Notch Signaling Pathway Research: Fundamental tool for studying the role of γ-secretase in cleaving transmembrane proteins like Notch and APP (Amyloid Precursor Protein).
• Alzheimer's Disease Models: Used in vitro and in vivo to inhibit Aβ peptide production, aiding in the study of amyloid plaque formation and potential therapeutic interventions.
• Cancer Biology Studies: Applied to investigate the oncogenic role of dysregulated Notch signaling in various cancers, including T-cell acute lymphoblastic leukemia (T-ALL) and breast cancer.
• Stem Cell & Developmental Biology: Utilized to modulate cell fate decisions and differentiation processes controlled by Notch signaling.
• High-Throughput Screening (HTS): Serves as a reference standard or active control in screening assays designed to discover novel γ-secretase modulators or inhibitors.
• Biochemical Assay Development: Key component in establishing and validating enzymatic assays for γ-secretase activity and inhibitor potency (IC50 determination).

Basic Information

Product Name	γ-Secretase Inhibitor Xxi
CAS No.	209986-17-4
Molecular Formula	C₂₃H₂₂F₂N₄O₃
Molecular Weight	440.44 g/mol
Synonyms	DAPT; N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester; LY-374973; GSI-IX; γ-Secretase Inhibitor IX; (2S)-N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl-1,1-dimethylethyl esterglycine; N2-[(2S)-2-[(1,1-Dimethylethoxy)carbonylamino]-1-oxopropyl]-N1-(3,5-difluorophenyl)-2-oxo-1-pyrrolidineacetamide
EINECS	Contact for details

Quality Control

Our γ-Secretase Inhibitor Xxi is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional activity assays. A detailed Certificate of Analysis (COA) providing full specifications and test results is supplied with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular ion [M+H]+
Purity (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.5%
Biological Activity (IC50)	≤20 nM (γ-Secretase inhibition in cell-based assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.