Description

D4 C27 Alpha Alpha Alpha (20R) Cholestane is a high-purity, deuterium-labeled sterane standard, specifically designed for analytical and research applications requiring precise isotopic labeling. This compound is critical for ensuring accuracy and reliability in quantitative mass spectrometry and metabolic studies, where it serves as an essential internal standard. It is primarily utilized by analytical laboratories, pharmaceutical researchers, and petrochemical geochemists for method development, calibration, and complex mixture analysis.

Application

• Internal Standard for Mass Spectrometry (MS): Used for the accurate quantification of cholesterol, sterols, and related biomarkers in biological and environmental samples via GC-MS or LC-MS.
• Metabolic Pathway Research: Acts as a stable isotopic tracer in studies of cholesterol biosynthesis, metabolism, and transport within biological systems.
• Pharmaceutical Analysis: Employed in the development and validation of analytical methods for sterol-based drug compounds and formulations.
• Geochemical Biomarker Analysis: Serves as a reference standard in petroleum geochemistry for the identification and quantification of sterane biomarkers in crude oils and sedimentary rocks.
• Quality Control in Clinical Diagnostics: Used to calibrate instruments and verify results in clinical testing laboratories measuring sterol panels.
• Academic and Industrial Research: A key reagent in synthetic chemistry and biochemistry research involving steroid and sterol molecular frameworks.

Basic Information

Product Name	D4 C27 Alpha Alpha Alpha (20R) Cholestane
CAS No.	205529-74-4
Molecular Formula	C₂₇D₄₆
Molecular Weight	374.71 g/mol (for D4 isotopologue)
Synonyms	Cholestane-d4; (20R)-Cholestane-d4; Deuterated Cholestane; 5α-Cholestane-d4; 20R-5α-Cholestane-d4; 5α,14α,17α,20R-Cholestane-d4; D4-5α-Cholestane; Isotopically Labeled Cholestane Standard
EINECS	Contact for details

Quality Control

Our D4 C27 Alpha Alpha Alpha (20R) Cholestane is manufactured under strict quality systems to ensure exceptional isotopic and chemical purity. Each batch is characterized using advanced analytical techniques including NMR, GC-MS, and HPLC to confirm identity, isotopic enrichment (typically >98 atom % D), and chemical purity. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. For long-term stability, consider storage at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white crystalline solid
Identification (IR / NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (Atom % D)	≥ 98.0%
Chemical Purity (GC)	≥ 99.0%
Specific Optical Rotation	Contact for details
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.