Description

5A-Pregnan-3A-Ol-20-One-D4 is a deuterium-labeled steroid derivative, specifically a deuterated analog of 5α-Pregnan-3α-ol-20-one (allopregnanolone). This compound is of significant value as a high-purity internal standard and reference material for analytical research, enabling precise quantification and metabolic studies. It is primarily needed by pharmaceutical R&D laboratories, contract research organizations (CROs), and academic institutions engaged in neurosteroid research, pharmacokinetics, and mass spectrometry-based bioanalysis.

Application

• Internal Standard for LC-MS/MS: Critical for the accurate quantification of endogenous allopregnanolone and related neurosteroids in biological matrices like plasma, serum, and cerebrospinal fluid.
• Metabolic Pathway Tracing: Used in deuterium tracing studies to investigate the biosynthesis, metabolism, and pharmacokinetic profiles of neuroactive steroids.
• Pharmaceutical Reference Standard: Serves as a certified reference material (CRM) for method development, validation, and quality control in drug discovery programs targeting GABA-A receptors.
• Biomarker Research: Facilitates research into neurological and psychiatric conditions, such as stress, depression, epilepsy, and premenstrual dysphoric disorder (PMDD), where allopregnanolone levels are implicated.
• Isotope Dilution Analysis: Employed in high-precision analytical techniques to correct for losses during sample preparation and instrument variability.

Basic Information

Product Name	5A-Pregnan-3A-Ol-20-One-D4
CAS No.	203805-85-0
Molecular Formula	C21H30D4O2
Molecular Weight	322.52 g/mol (approx.)
Synonyms	Allopregnanolone-d4; 3α-Hydroxy-5α-pregnan-20-one-d4; 5α-Pregnan-3α-ol-20-one-d4; (3α,5α)-3-Hydroxypregnan-20-one-d4; THP-d4; 3α,5α-THP-d4; Deuterated Allopregnanolone; 5α-Pregnan-3α-ol-20-one-2,2,4,4-d4
EINECS	Contact for details

Quality Control

Our deuterated standards are produced under strict quality control to ensure the highest isotopic purity and chemical integrity, essential for reliable analytical results. Each batch is characterized using advanced techniques including NMR, HPLC, and high-resolution mass spectrometry. A comprehensive Certificate of Analysis (COA) is provided, detailing isotopic enrichment (typically ≥98 atom % D), chemical purity (by HPLC), and spectroscopic confirmation. We support compliance with GLP and ISO 17025 standards for reference materials.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (refrigerated) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation or storage under an inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Purity	≥98 atom % D
Chemical Purity	≥95%
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.