Description

L-Proline-13C5 is a stable isotope-labeled analog of the amino acid L-proline, where all five carbon atoms are the 13C isotope. This compound is essential for advanced quantitative proteomics, metabolic flux analysis, and pharmaceutical research requiring precise internal standards for mass spectrometry. It is a critical reagent for scientists and manufacturers in the fields of isotope labeling, drug metabolism and pharmacokinetics (DMPK) studies, and nutritional research.

Application

• Mass Spectrometry Internal Standard: Used as a quantitative reference standard in LC-MS and GC-MS analyses for accurate measurement of endogenous proline levels in biological samples.
• Metabolic Pathway Tracing (Fluxomics): Employed in 13C metabolic flux analysis to map and quantify the flow of carbon through biochemical networks in cells, tissues, or organisms.
• Pharmaceutical R&D: Critical for drug metabolism studies (DMPK) to track the incorporation of labeled atoms into drug molecules or metabolites.
• Proteomics Research: Facilitates the development of stable isotope labeling by amino acids in cell culture (SILAC) and other quantitative proteomic techniques.
• Nutritional Science: Used in studies investigating collagen synthesis, wound healing, and amino acid metabolism in vivo.
• Diagnostic Reagent Manufacturing: Serves as a key raw material in the production of high-precision clinical diagnostic kits and calibrators.

Basic Information

Product Name	L-Proline-13C5
CAS No.	201740-83-2
Molecular Formula	13C5H9NO2
Molecular Weight	120.10 (based on 13C)
Synonyms	(2S)-Pyrrolidine-2-carboxylic acid-13C5; L-(-)-Proline-13C5; Proline-13C5; (S)-Pyrolidine-2-carboxylic acid-13C5; (S)-Proline-13C5; (-)-Proline-13C5; 13C5-L-Proline; Isotopically labeled L-proline
EINECS	Contact for details

Quality Control

Our L-Proline-13C5 is manufactured under strict quality systems to ensure the highest isotopic and chemical purity for research and diagnostic applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (Atom % 13C), chemical purity (by HPLC), chiral purity, and spectroscopic confirmation. We support compliance with relevant GMP and ISO 13485 standards for regulated applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C for long-term stability. This material is hygroscopic (moisture-sensitive); ensure the container is sealed under inert atmosphere after each use to prevent moisture uptake and decomposition.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 99 atom % 13C
Chiral Purity ([α]D)	≥ 99.0% (L-isomer)
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.