Description

L-Phenylalanine-13C9, 15N is a stable isotope-labeled analog of the essential amino acid L-Phenylalanine, where all nine carbon atoms are the 13C isotope and the nitrogen atom is the 15N isotope. This compound is critical for advanced research and analytical applications requiring precise metabolic tracing and quantification. It is primarily utilized by scientists and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries for applications such as metabolic pathway analysis, proteomics, and the development of internal standards for mass spectrometry.

Application

• Mass Spectrometry Internal Standards: Serves as a critical quantitative reference standard in LC-MS and GC-MS analyses for accurate measurement of endogenous phenylalanine levels in biological samples.
• Metabolic Flux Analysis (MFA): Used as a tracer to study the dynamics of phenylalanine metabolism in cell cultures, tissues, and whole organisms, providing insights into metabolic disorders and disease mechanisms.
• Proteomics & Protein Labeling: Employed in Stable Isotope Labeling by Amino acids in Cell culture (SILAC) for quantitative, large-scale protein analysis and turnover studies.
• Pharmaceutical Research: Facilitates the study of drug metabolism and pharmacokinetics (DMPK) for compounds that interact with phenylalanine pathways or are phenylalanine derivatives.
• Nutritional Science Research: Enables precise tracking of phenylalanine utilization and protein synthesis in nutritional studies and clinical research.
• Diagnostic Assay Development: Used in the manufacturing and calibration of high-precision in-vitro diagnostic (IVD) kits for conditions like phenylketonuria (PKU).

Basic Information

Product Name	L-Phenylalanine-13C9, 15N
CAS No.	29700-34-3
Molecular Formula	13C9C2H11NO2 or 13C915N-C9H11NO2
Molecular Weight	~188.15 g/mol (based on isotopic composition)
Synonyms	(2S)-2-Amino-3-phenylpropanoic acid-13C9,15N; Phenylalanine-13C9,15N; L-Phe-13C9,15N; (S)-Phenylalanine-13C9,15N; F-13C9,15N; U-13C9,15N-L-Phenylalanine; Phenylalanine (13C9, 15N); Isotopically labeled L-Phenylalanine
EINECS	Contact for details

Quality Control

Every batch of L-Phenylalanine-13C9, 15N is manufactured and analyzed under strict quality systems to ensure exceptional isotopic and chemical purity, critical for sensitive research applications. Our quality commitment includes comprehensive testing for isotopic enrichment (by NMR or MS), chemical purity (by HPLC), chiral purity, and verification of structure. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting the specific results for key parameters such as assay, isotopic purity, and optical rotation.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive). Keep the container tightly sealed in a dry environment to prevent absorption of moisture, which can affect stability and accurate weighing.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0% (on anhydrous basis)
Isotopic Purity	≥ 99 atom % 13C; ≥ 99 atom % 15N
Optical Rotation	-33.0° to -35.0° (c=1 in H2O)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.