Description

Phenylthiohydantoin Isoleucine is a specialized derivative of isoleucine, a critical amino acid, where the phenylthiohydantoin (PTH) group is attached. This compound is essential for advanced biochemical analysis, particularly in protein sequencing and peptide mapping via Edman degradation. It serves as a key analytical standard and reagent for research institutions, pharmaceutical developers, and biotechnology companies engaged in proteomics and high-purity amino acid analysis.

Application

• Protein Sequencing Reagent: Critical component in the Edman degradation process for stepwise determination of amino acid sequences in peptides and proteins.
• Analytical Reference Standard: Used for calibration and validation in High-Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) systems for amino acid analysis.
• Biochemical Research: Employed in proteomics research to study protein structure, function, and post-translational modifications.
• Pharmaceutical Development: Utilized in the characterization and quality control of peptide-based therapeutics and biologics.
• Diagnostic Kit Manufacturing: A key raw material in the production of kits for amino acid and metabolic disorder diagnostics.

Basic Information

Product Name	Phenylthiohydantoin Isoleucine
CAS No.	29583-07-1
Molecular Formula	C13H16N2O2S
Molecular Weight	264.34 g/mol
Synonyms	PTH-Isoleucine; 3-Phenyl-2-thiohydantoin isoleucine; Isoleucine phenylthiohydantoin; (2S,3S)-2-Amino-3-methylpentanoic acid phenylthiohydantoin derivative; Edman degradation product of Isoleucine; 5-Isobutyl-3-phenyl-2-thioxoimidazolidin-4-one; Ile-PTH
EINECS	Contact for details

Quality Control

Our Phenylthiohydantoin Isoleucine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and analytical applications. A Certificate of Analysis (COA) detailing purity, chromatographic data, and physical properties is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Solubility	Soluble in common organic solvents (e.g., acetonitrile, methanol)
Loss on Drying	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.