Description

Phorbol 12,13-Dibenzoate is a potent, cell-permeable diacylglycerol (DAG) analog and a well-characterized protein kinase C (PKC) activator. This compound is a critical research tool for studying signal transduction pathways, cellular proliferation, and tumor promotion mechanisms. It is primarily utilized by pharmaceutical researchers, academic institutions, and biotechnology companies engaged in cancer research, immunology, and molecular biology.

Application

• Biomedical Research: As a standard PKC activator for studying enzyme kinetics, signal transduction cascades, and second messenger systems in vitro.
• Cancer Research: Used to investigate tumor promotion mechanisms, cell transformation, and the role of PKC isoforms in various cancer cell lines.
• Immunology Studies: Employed to activate T-cells, B-cells, and other immune cells to study cytokine production, cell activation, and immune response modulation.
• Pharmacology & Toxicology: Serves as a reference compound in assays screening for PKC inhibitors or modulators for drug discovery.
• Cell Biology: Applied in studies of cellular differentiation, apoptosis, and gene expression regulated by the PKC pathway.
• Biochemical Assay Standard: Used as a high-purity calibrant and positive control in enzymatic and binding assays targeting PKC.

Basic Information

Product Name	Phorbol 12,13-Dibenzoate
CAS No.	25405-85-0
Molecular Formula	C36H38O8
Molecular Weight	598.69 g/mol
Synonyms	PDBu; Phorbol 12,13-Dibenzate; 12,13-Dibenzoylphorbol; 4,9,12-beta,13-alpha,20-Pentahydroxytiglia-1,6-dien-3-one 12,13-dibenzoate; NSC 169640; 12-O,13-O-Dibenzoylphorbol; 12,13-Di-O-benzoylphorbol; TPA analog PDBu
EINECS	Contact for details

Quality Control

Our Phorbol 12,13-Dibenzoate is produced under strict quality management protocols to ensure batch-to-batch consistency and high chemical purity suitable for sensitive research applications. Each lot is characterized by advanced analytical techniques, including HPLC and NMR, to confirm identity and potency. A comprehensive Certificate of Analysis (COA) detailing purity, assay results, and analytical methods is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under inert atmosphere for long-term stability. The product is light-sensitive (store away from light). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%
Solubility	Soluble in DMSO, ethanol, acetone; insoluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.