Description

Thymine, [2-14C] is a carbon-14 labeled isotopologue of the nucleobase thymine, a fundamental component of DNA. This radiolabeled compound is essential for conducting precise metabolic and pharmacokinetic studies, enabling researchers to track the incorporation and fate of thymine within biological systems. It is a critical reagent for scientists in pharmaceutical development, biochemistry research, and molecular biology, particularly for investigating nucleic acid synthesis, DNA repair mechanisms, and cell proliferation.

Application

• Tracer Studies in Drug Metabolism and Pharmacokinetics (DMPK): Used to label and trace the metabolic pathways of novel pharmaceutical compounds that interact with or are analogs of thymine.
• DNA/RNA Synthesis and Repair Research: Employed in autoradiography and liquid scintillation counting to study the kinetics of DNA replication, repair processes, and nucleotide pool dynamics.
• Biochemical Pathway Analysis: Facilitates the elucidation of thymine and thymidine salvage pathways in various cell types and under different physiological conditions.
• Development of Diagnostic Assays: Serves as a key component in the development of sensitive radioimmunoassays (RIAs) or other detection methods for enzymes involved in nucleotide metabolism.
• Cancer and Cell Proliferation Research: Used to measure cell proliferation rates and the effects of chemotherapeutic agents on DNA synthesis in vitro and in vivo.

Basic Information

Product Name	Thymine, [2-14C]
CAS No.	24523-96-4
Molecular Formula	C5H614CN2O2
Molecular Weight	~128.1 g/mol (exact mass depends on isotopic enrichment)
Synonyms	5-Methyl[2-14C]uracil; 2,4-Dihydroxy-5-methyl[2-14C]pyrimidine; [2-14C]Thymine; 5-Methyl-2,4(1H,3H)-pyrimidinedione-2-14C; 14C-Thymine; Radiolabeled Thymine; Carbon-14 Labeled Thymine
EINECS	Contact for details

Quality Control

Every batch of Thymine, [2-14C] is manufactured and analyzed under strict quality systems to ensure high radiochemical purity, chemical purity, and specific activity. Our quality control protocols are designed to meet the stringent requirements of research and development laboratories. Comprehensive Certificates of Analysis (COA) detailing radiochemical purity (by HPLC), chemical purity, specific activity, and solvent composition are provided with each shipment to ensure full traceability and compliance with your experimental protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider storage at -20°C under inert atmosphere. Handle and dispose of in accordance with all applicable local, national, and international radiation safety regulations.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to reference standard
Radiochemical Purity (HPLC)	≥ 97%
Chemical Purity (HPLC)	≥ 98%
Specific Activity	50-60 mCi/mmol (Contact for specific value)
Solvent (if supplied in solution)	Ethanol:Water (Specified on COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.