Description

Leupeptin CAS NO 24365-47-7 is a potent, cell-permeable protease inhibitor that specifically targets serine, cysteine, and threonine proteases. This compound is a critical research tool for studying proteolytic pathways, preventing unwanted protein degradation in cell lysates and tissue extracts. It is essential for researchers and manufacturers in pharmaceutical development, biochemistry, and molecular biology, where precise control of protease activity is required for accurate experimental and production outcomes.

Application

• Biochemical Research: Used as a standard protease inhibitor in cell lysis buffers to preserve protein integrity during extraction and purification.
• Pharmaceutical R&D: Employed in studies of disease mechanisms involving proteases, such as cancer metastasis, inflammation, and neurodegenerative disorders.
• Diagnostic Reagent Production: Incorporated into diagnostic kit formulations to stabilize protein components and ensure assay reliability.
• Enzyme Characterization: Serves as a specific inhibitor to study the activity and function of various serine and cysteine proteases in vitro.
• Cell Culture & Tissue Engineering: Added to media to inhibit extracellular matrix degradation, aiding in the maintenance of cell structure and function.
• Food Science Research: Applied in studies of enzymatic spoilage and texture changes in food products to develop preservation strategies.

Basic Information

Product Name	Leupeptin
CAS No.	24365-47-7
Molecular Formula	C₂₀H₃₈N₆O₄
Molecular Weight	426.56 g/mol
Synonyms	Acetyl-Leu-Leu-Arg-al; N-Acetyl-L-leucyl-L-leucyl-L-arginal; Leupeptin Hemisulfate; Protease Inhibitor Leupeptin; (2S)-1-[(2S)-2-Acetamido-4-methylpentanoyl]-N-[(3S)-5-(diaminomethylideneamino)-1-oxo-1-pentan-3-yl]pyrrolidine-2-carboxamide
EINECS	Contact for details

Quality Control

Our Leupeptin is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, identity confirmation (IR), and moisture content analysis, to ensure it meets high-purity research standards. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.