Description

Choline Chloride, [Methyl-14C] is a radiolabeled form of the essential nutrient choline chloride, where the methyl group is tagged with the carbon-14 isotope. This specific labeling is critical for conducting precise metabolic and pharmacokinetic studies, enabling researchers to track the compound's fate in biological systems with high sensitivity. It is an indispensable tool for scientists in pharmaceutical development, nutritional biochemistry, and agricultural research who require accurate tracer methodologies.

Application

• Metabolic Pathway Tracing: Used to study the biosynthesis and turnover of phosphatidylcholine, betaine, and acetylcholine in vivo and in vitro.
• Drug Metabolism and Pharmacokinetics (DMPK): Essential for ADME (Absorption, Distribution, Metabolism, Excretion) studies of new drug candidates that interact with cholinergic pathways or lipid metabolism.
• Nutritional Research: Investigates choline uptake, utilization, and requirements in animal models and cell cultures.
• Agricultural Science: Employed in studies on choline's role as a feed additive and its metabolism in livestock.
• Biochemical Assay Development: Serves as a labeled substrate or standard in enzymatic assays for choline kinase and other related enzymes.
• Environmental Fate Studies: Tracks the degradation and environmental persistence of choline-based compounds.

Basic Information

Product Name	Choline Chloride, [Methyl-14C]
CAS No.	24333-16-2
Molecular Formula	C5H14ClN*O (14C labeled)
Molecular Weight	~139.62 g/mol (unlabeled)
Synonyms	(2-Hydroxyethyl)trimethylammonium Chloride, [Methyl-14C]; Biocolina; Hepacholine; 14C-Choline Chloride; Choline Chloride (14C-methyl); Lipotril; (β-Hydroxyethyl)trimethylammonium Chloride, [14C]methyl-; Vitamin B4 Chloride (labeled)
EINECS	Contact for details

Quality Control

Every batch of Choline Chloride, [Methyl-14C] is manufactured and analyzed under strict quality systems to ensure traceability and reliability for research. Product identity, radiochemical purity (>97% typical), and specific activity are rigorously confirmed via techniques including HPLC with radiometric detection and NMR spectroscopy. A comprehensive Certificate of Analysis (COA) detailing batch-specific data—including radioactive concentration, solvent, and expiration date—is provided with each shipment to support regulatory submissions and ensure experimental reproducibility.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C or as specified on the label. The product is sensitive to moisture and should be kept in a cool, dry place. Users must adhere to all local and international regulations for the safe handling and storage of radioactive materials.

Specification

Item	Specification
Appearance	White to off-white crystalline solid or solution (as specified)
Identification (IR/NMR)	Conforms to structure
Assay (HPLC)	≥ 95% (Chemical Purity)
Radiochemical Purity (HPLC)	≥ 97%
Specific Activity	50-60 mCi/mmol (Batch Specific)
Solvent (if supplied as solution)	Ethanol:Water or as specified

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.