Description

Leupeptin Acid CAS NO 24125-28-8 is a potent, naturally occurring protease inhibitor that specifically targets serine, cysteine, and threonine proteases. This compound is a critical research tool for studying proteolytic pathways and cellular processes, offering high specificity and reversible inhibition. It is essential for scientists and manufacturers in biomedical research, pharmaceutical development, and biochemical production where precise control of protease activity is required.

Application

• Biochemical Research: Used as a standard inhibitor to study the function of proteases like trypsin, plasmin, and papain in enzymatic assays and signal transduction pathways.
• Cell Culture & Protein Purification: Added to cell lysis buffers and purification protocols to prevent unwanted protein degradation and preserve target protein integrity.
• Pharmaceutical R&D: Serves as a key reagent in drug discovery programs targeting protease-involved diseases, such as cancer, inflammation, and viral infections.
• Diagnostic Kit Manufacturing: Employed as a stabilizing agent in diagnostic reagents and kits to ensure consistent enzyme activity and assay performance.
• Apoptosis & Neuroscience Studies: Utilized to investigate the role of calpains and caspases in programmed cell death and neuronal function.

Basic Information

Item	Details
Product Name	Leupeptin Acid
CAS No.	24125-28-8
Molecular Formula	C₂₀H₃₈N₆O₄
Molecular Weight	426.55 g/mol
Synonyms	Leupeptin; Acetyl-Leu-Leu-Arg-al; N-Acetyl-L-leucyl-L-leucyl-L-arginal; Leupeptin Hemisulfate; Protease Inhibitor Leupeptin; (2S)-1-[(2S)-2-Acetamido-4-methylpentanoyl]-N-[(1S)-4-[(diaminomethylidene)amino]-1-formylbutyl]pyrrolidine-2-carboxamide
EINECS	Contact for details

Quality Control

Our Leupeptin Acid is produced under stringent quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification. We provide full traceability and Certificates of Analysis (COA) are available upon request, ensuring compliance with research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content	≤ 5.0%
Solubility	Soluble in water, DMSO, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.