Description

Leupeptin Hydrochloride CAS NO 24125-16-4 is a potent, cell-permeable serine and cysteine protease inhibitor widely used in biochemical and cell biology research. This compound is critical for preventing protein degradation during cell lysis and protein purification, thereby ensuring the integrity of experimental results. It is an essential reagent for researchers in pharmaceutical development, academic institutions, and biotechnology companies working on apoptosis, signal transduction, and inflammation pathways.

Application

• Protease Inhibition in Cell Lysates: Prevents unwanted proteolytic degradation during the extraction of proteins, enzymes, and nucleic acids from tissues and cultured cells.
• Apoptosis & Signal Transduction Research: Used to study caspase activity and other protease-mediated pathways involved in programmed cell death and cellular signaling.
• Protein Purification & Stabilization: Added to buffers to maintain the stability and activity of sensitive proteins throughout purification protocols like affinity chromatography.
• Inflammation & Immunology Studies: Investigates the role of proteases like calpain and cathepsins in immune cell function and inflammatory responses.
• Enzyme Kinetic Assays: Serves as a specific inhibitor to characterize the activity and substrate specificity of various serine and cysteine proteases.
• Biopharmaceutical Production: Employed in upstream and downstream processing to control protease activity and improve the yield of recombinant therapeutic proteins.

Basic Information

Product Name	Leupeptin Hydrochloride
CAS No.	24125-16-4
Molecular Formula	C₂₀H₃₈N₆O₄ • HCl
Molecular Weight	463.0 g/mol
Synonyms	Leupeptin Hemisulfate; Acetyl-Leu-Leu-Arg-al; N-Acetyl-L-leucyl-L-leucyl-L-argininal Hydrochloride; Leupeptin HCl; Protease Inhibitor Leupeptin; (2S)-1-[(2S)-2-Acetamido-4-methylpentanoyl]-N-[(1S)-4-[(diaminomethylidene)amino]-1-formylbutyl]pyrrolidine-2-carboxamide hydrochloride
EINECS	Contact for details

Quality Control

Our Leupeptin Hydrochloride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by spectroscopic methods, and stringent controls for moisture and related substances. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant and under inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm
Biological Activity	Tested in protease inhibition assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.