Description

20-Hydroperoxycholesterol is a significant oxidized derivative of cholesterol, serving as a key intermediate in the biosynthesis of steroid hormones and oxysterols. Its primary value lies in its role as a research chemical for studying oxidative stress pathways, lipid peroxidation, and cholesterol metabolism. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors, particularly those focused on metabolic disease research, drug discovery, and the development of analytical standards.

Application

• Biochemical Research: Critical intermediate for investigating the enzymatic and non-enzymatic oxidation pathways of cholesterol.
• Pharmaceutical Development: Used as a reference standard and precursor in studies targeting atherosclerosis, neurodegenerative diseases, and other conditions linked to oxidative stress.
• Analytical Standard: Serves as a high-purity calibrant and internal standard in chromatographic (HPLC, GC) and mass spectrometric analyses for lipidomics.
• Metabolic Pathway Studies: Employed to elucidate the biological activity and signaling roles of specific oxysterols in cell culture and animal models.
• Enzyme Substrate: Utilized in assays for enzymes involved in sterol metabolism, such as specific cytochrome P450 isoforms.

Basic Information

Product Name	20-Hydroperoxycholesterol
CAS No.	23653-01-2
Molecular Formula	C27H46O3
Molecular Weight	418.66 g/mol
Synonyms	20(S)-Hydroperoxycholesterol; 20-Hydroperoxycholest-5-en-3β-ol; Cholest-5-ene-3β,20-diol 20-hydroperoxide; 20α-Hydroperoxycholesterol; 20-HpCh; 20(S)-HP-Chol; (3β,20S)-Cholest-5-ene-3,20-diol 20-hydroperoxide
EINECS	Contact for details

Quality Control

Our 20-Hydroperoxycholesterol is produced and handled under strict quality protocols to ensure batch-to-batch consistency and high purity for research applications. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and MS to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent degradation. The material is easily oxidized and light-sensitive; allow it to reach room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 90% (by titration)
Solubility	Soluble in chloroform, DMSO, ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.