Description

ε-Amanitin is a highly potent bicyclic octapeptide belonging to the amatoxin family, isolated from certain mushroom species. This compound is of critical importance in biochemical and pharmacological research due to its specific and irreversible inhibition of RNA polymerase II, a key enzyme in eukaryotic transcription. It is primarily utilized by research institutions, pharmaceutical developers, and toxicology laboratories for studying transcription mechanisms, developing inhibitors, and as a reference standard in toxin analysis.

Application

• Biochemical Research: As a potent and selective inhibitor of RNA polymerase II for studying eukaryotic gene transcription, mRNA synthesis, and transcriptional regulation.
• Pharmacology & Toxicology: Used as a reference standard and tool compound in mechanistic studies of hepatotoxicity, cell death pathways (apoptosis), and for the development of potential antidotes.
• Diagnostic Development: Serves as a critical calibrant and control in the development of highly sensitive analytical methods (e.g., LC-MS/MS, ELISA) for the detection of amatoxins in biological and environmental samples.
• Cell Biology Studies: Employed to induce transcriptional arrest in cell culture models to investigate protein turnover, stress responses, and the role of nascent RNA in cellular processes.
• Forensic Science: Used as an analytical standard for the definitive identification and quantification of amatoxin poisoning in forensic investigations.

Basic Information

Product Name	ε-Amanitin
CAS No.	21705-02-2
Molecular Formula	C39H54N10O14S
Molecular Weight	918.98 g/mol
Synonyms	ε-Amanitin; ε-Amanitine; Amaninamide; (3S,6S,9S,12S,15S,18S,21S,24S,30S)-30-[(1S)-1-Hydroxyethyl]-21-(2-hydroxyethyl)-3,6,9,12,15,18,24-heptamethyl-1,4,7,10,13,16,19,22,25,28-decaoxo-2,5,8,11,14,17,20,23,26,29-decaazatricyclo[29.3.0.012,16]tetratriacontane-30-carboxamide; NSC 119065; Amatoxin ε
EINECS	Contact for details

Quality Control

Our ε-Amanitin is produced and handled under strict quality-controlled conditions suitable for research applications. Each batch is characterized using advanced analytical techniques including HPLC, MS, and NMR to ensure identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and related substance profiles is provided with every shipment to guarantee traceability and consistency for your critical research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to prevent condensation. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in methanol, DMSO; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.