Description

4-Nitrophenyl 4'-Guanidinobenzoate is a specialized organic compound that serves as a key biochemical tool and synthetic intermediate. Its primary value lies in its role as a chromogenic substrate for the detection and study of serine proteases, particularly trypsin and related enzymes. This makes it an essential reagent for researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic industries who require precise enzyme activity measurement and inhibition studies.

Application

• Biochemical Research: As a chromogenic substrate for the spectrophotometric assay of trypsin, thrombin, and other serine proteases.
• Pharmaceutical Development: Used in high-throughput screening (HTS) for the discovery and evaluation of novel protease inhibitor drug candidates.
• Diagnostic Reagent Manufacturing: A critical component in the formulation of diagnostic kits for measuring pancreatic function and other clinical protease levels.
• Enzyme Kinetics Studies: Employed to determine kinetic parameters (Km, Vmax) and study enzyme mechanism and specificity.
• Quality Control in Bioprocessing: Monitoring protease activity during upstream and downstream biomanufacturing processes.
• Academic Research: A standard tool in university and institutional labs for teaching and investigating proteolytic pathways.

Basic Information

Product Name	4-Nitrophenyl 4'-Guanidinobenzoate
CAS No.	21658-26-4
Molecular Formula	C14H12N4O4
Molecular Weight	300.27 g/mol
Synonyms	4-Guanidinobenzoic Acid 4-Nitrophenyl Ester; p-Guanidinobenzoic Acid p-Nitrophenyl Ester; NSC 113992; 4-Nitrophenyl p-Guanidinobenzoate; Trypsin Substrate (Chromogenic); Bz-Arg-OPhNO2 (common abbreviation); Benzoyl-L-arginine p-nitroanilide analog (structural analog); N-α-Benzoyl-L-arginine 4-nitroanilide hydrochloride related compound
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl 4'-Guanidinobenzoate is produced under strict quality management systems to ensure batch-to-batch consistency and reliability for sensitive biochemical applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by spectroscopic methods. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting critical parameters such as assay, impurity profile, and physical characteristics to meet the stringent requirements of research and diagnostic manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive); the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Loss on Drying	≤ 1.0%
Heavy Metals	< 20 ppm
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Validated as a substrate for trypsin

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.